ArticleFrontiers in plant science2015
Factors influencing somatic embryogenesis, regeneration, and Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) cultivar TME14.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- Optimizing somatic embryogenesis in Indonesian cassava genotypes: Effects of 2,4-D or Picloram supplementation and explant type.Plant biotechnology (Tokyo, Japan) · 2026Article
- Advancements and strategies of genetic improvement in cassava (Horticulture research · 2025Article
- Improving gene editing of CRISPR/Cas9 using the callus-specific promoterFrontiers in plant science · 2025Article
- An Anthocyanin-Based Visual Reporter System for Genetic Transformation and Genome Editing in Cassava.International journal of molecular sciences · 2024Article
- Expression Pattern and Functional Analysis ofPlants (Basel, Switzerland) · 2024Article
- Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber.Scientific reports · 2024Article
- Exploring Agrobacterium-mediated genetic transformation methods and its applications in Lilium.Plant methods · 2024Review
- Effect of gamma irradiation on proliferation and growth of friable embryogenic callus andFrontiers in plant science · 2024Article
- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
- Article
- Agrobacterium-mediated cassava transformation for the Asian elite variety KU50.Plant molecular biology · 2022Article
- Inorganic Compounds that Aid in Obtaining Somatic Embryos.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Efficient Genetic Transformation and Regeneration of a Farmer-Preferred Cassava Cultivar From Ghana.Frontiers in plant science · 2021Article
- Establishment of Efficient Genetic Transformation Systems and Application of CRISPR/Cas9 Genome Editing Technology inInternational journal of molecular sciences · 2019Article
- Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels.Methods and protocols · 2018Article
- Overexpression of rice thaumatin-like protein (Journal, genetic engineering & biotechnology · 2018Article
- Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz).BMC biotechnology · 2017Article
- Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.PloS one · 2017Article
- MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds.Scientific reports · 2016Article
- Transition from somatic embryo to friable embryogenic callus in cassava: dynamic changes in cellular structure, physiological status, and gene expression profiles.Frontiers in plant science · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Routine production of large numbers of transgenic plants is required to fully exploit advances in cassava biotechnology and support development of improved germplasm for deployment to farmers. This article describes an improved, high-efficiency transformation protocol for recalcitrant cassava cultivar TME14 preferred in Africa. Factors that favor production of friable embryogenic calli (FEC) were found to be use of DKW medium, crushing of organized embryogenic structures (OES) through 1-2 mm sized metal wire mesh, washing of crushed OES tissues and short exposure of tyrosine to somatic embryos; and transformation efficiency was enhanced by use of low Agrobacterium density during co-cultivation, co-centrifugation of FEC with Agrobacterium, germination of paramomycin resistant somatic embryos on medium containing BAP with gradual increase in concentration and variations of the frequency of subculture of cotyledonary-stage embryos on shoot elongation medium. By applying the optimized parameters, FEC were produced for cassava cultivar TME14 and transformed using Agrobacterium strain LBA4404 harboring the binary vector pCAMBIA2301. About 70-80 independent transgenic lines per ml settled cell volume (SCV) of FEC were regenerated on selective medium. Histochemical GUS assays confirmed the expression of gusA gene in transformed calli, somatic embryos and transgenic plants. The presence and integration of the gusA gene were confirmed by PCR and Southern blot analysis, respectively. RT-PCR analysis of transgenic plants confirmed the expression of gusA gene. This protocol demonstrates significantly enhanced transformation efficiency over existing cassava transformation protocols and could become a powerful tool for functional genomics and transferring new traits into cassava.
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