ArticleFrontiers in synaptic neuroscience2015
Electron microscopic tomography reveals discrete transcleft elements at excitatory and inhibitory synapses.
Article in Frontiers in synaptic neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Transsynaptic Assemblies Link Domains of Presynaptic and Postsynaptic Intracellular Structures across the Synaptic Cleft.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Designer molecules of the synaptic organizer MDGA1 reveal 3D conformational control of biological function.The Journal of biological chemistry · 2023Article
- Modification of the synaptic cleft under excitatory conditions.Frontiers in synaptic neuroscience · 2023Article
- Cryo-EM tomography and automatic segmentation delineate modular structures in the postsynaptic density.Frontiers in synaptic neuroscience · 2023Article
- The Structural Basis of Long-Term Potentiation in Hippocampal Synapses, Revealed by Electron Microscopy Imaging of Lanthanum-Induced Synaptic Vesicle Recycling.Frontiers in cellular neuroscience · 2022Article
- The Nanoscopic Organization of Synapse Structures: A Common Basis for Cell Communication.Membranes · 2021Review
- Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Comparative 2D and 3D Ultrastructural Analyses of Dendritic Spines from CA1 Pyramidal Neurons in the Mouse Hippocampus.International journal of molecular sciences · 2021Article
- Highly Conserved Molecular Features in IgLONs Contrast Their Distinct Structural and Biological Outcomes.Journal of molecular biology · 2020Article
- Postsynaptic protein organization revealed by electron microscopy.Current opinion in structural biology · 2019Review
- Neurexins - versatile molecular platforms in the synaptic cleft.Current opinion in structural biology · 2019Review
- Latrophilin's Social Protein Network.Frontiers in neuroscience · 2019Review
- Subsynaptic Domains in Super-Resolution Microscopy: The Treachery of Images.Frontiers in molecular neuroscience · 2019Review
- Article
- Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer.Journal of molecular biology · 2018Article
- Netrin-1 Promotes Synaptic Formation and Axonal Regeneration via JNK1/c-Jun Pathway after the Middle Cerebral Artery Occlusion.Frontiers in cellular neuroscience · 2018Article
- Identification of PSD-95 in the Postsynaptic Density Using MiniSOG and EM Tomography.Frontiers in neuroanatomy · 2018Article
- Transcellular Nanoalignment of Synaptic Function.Neuron · 2017Review
- A novel synaptic junction preparation for the identification and characterization of cleft proteins.PloS one · 2017Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
Electron microscopy has revealed an abundance of material in the clefts of synapses in the mammalian brain, and the biochemical and functional characteristics of proteins occupying synaptic clefts are well documented. However, the detailed spatial organization of the proteins in the synaptic clefts remains unclear. Electron microscope tomography provides a way to delineate and map the proteins spanning the synaptic cleft because freeze substitution preserves molecular details with sufficient contrast to visualize individual cleft proteins. Segmentation and rendering of electron dense material connected across the cleft reveals discrete structural elements that are readily classified into five types at excitatory synapses and four types at inhibitory synapses. Some transcleft elements resemble shapes and sizes of known proteins and could represent single dimers traversing the cleft. Some of the types of cleft elements at inhibitory synapses roughly matched the structure and proportional frequency of cleft elements at excitatory synapses, but the patterns of deployments in the cleft are quite different. Transcleft elements at excitatory synapses were often evenly dispersed in clefts of uniform (18 nm) width but some types show preference for the center or edges of the cleft. Transcleft elements at inhibitory synapses typically were confined to a peripheral region of the cleft where it narrowed to only 6 nm wide. Transcleft elements in both excitatory and inhibitory synapses typically avoid places where synaptic vesicles attach to the presynaptic membrane. These results illustrate that elements spanning synaptic clefts at excitatory and inhibitory synapses consist of distinct structures arranged by type in a specific but different manner at excitatory and inhibitory synapses.
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