ArticleMutagenesis2015
Cytotoxicity and genotoxicity of urban particulate matter in mammalian cells.
Article in Mutagenesis, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 59 citations in OpenAlex.
- Hemoglobin as a mediator between air pollution and growth outcomes in children under 60 months: the moderating role of nutritional supplementation.BMC public health · 2025Article
- Particulate Matter Induces Oxidative Stress and Ferroptosis in Human Lung Epithelial Cells.Toxics · 2024Article
- Impact of diesel exhaust particles on infections with Mycobacterium bovis BCG in in vitro human macrophages and an in vivo Galleria mellonella model.Environmental pollution (Barking, Essex : 1987) · 2024Article
- Geochemical compositional controls on DNA strand breaks induced in in vitro cell-free assays by crushed rock powders from the Panasqueira mine area, Portugal.Environmental geochemistry and health · 2021Article
- Genotoxic Activity of Particulate Matter and In Vivo Tests in Children Exposed to Air Pollution.International journal of environmental research and public health · 2021Article
- Determination of Genotoxicity Attributed to Diesel Exhaust Particles in Normal Human Embryonic Lung Cell (WI-38) Line.Biomolecules · 2021Article
- The marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men.Scientific reports · 2021Article
- Cellular effects of PMEnvironmental science and pollution research international · 2021Article
- Particulate Air Pollution and Osteoporosis: A Systematic Review.Risk management and healthcare policy · 2021Review
- Sulforaphane Alleviates Particulate Matter-Induced Oxidative Stress in Human Retinal Pigment Epithelial Cells.Frontiers in medicine · 2021Article
- Application of various cytotoxic endpoints for the toxicity prioritization of fine dust (PM2.5) sources using a multi-criteria decision-making approach.Environmental geochemistry and health · 2020Article
- Comet Test in Saliva Leukocytes of Pre-School Children Exposed to Air Pollution in North Italy: The Respira Study.International journal of environmental research and public health · 2020Article
- Fifteen Years of Airborne ParticulatesInternational journal of molecular sciences · 2020Review
- Application of a Quartz Crystal Microbalance to Measure the Mass Concentration of Combustion Particle Suspensions.Journal of aerosol science · 2019Article
- Can Plant Phenolic Compounds Protect the Skin from Airborne Particulate Matter?Antioxidants (Basel, Switzerland) · 2019Review
- Cytoprotective Effects of Mangiferin andNutrients · 2019Article
- Effect of collection methods on combustion particle physicochemical properties and their biological response in a human macrophage-like cell line.Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering · 2019Article
- Fine particulate matter (PMChronic diseases and translational medicine · 2018Article
- Dust at Various Workplaces-Microbiological and Toxicological Threats.International journal of environmental research and public health · 2018Article
- ComparativeToxicological research · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Ambient air particulate matter (PM)-associated reactive oxygen species (ROS) have been linked to a variety of altered cellular outcomes. In this study, three different PM samples from diesel exhaust particles (DEPs), urban dust standard reference material SRM1649a and air collected in Manchester have been tested for their ability to oxidise DNA in a cell-free assay, to increase intracellular ROS levels and to induce CYP1A1 gene expression in mammalian cells. In addition, the cytotoxicity and genotoxicity of PM were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and alkaline comet assay, respectively. All PM samples catalysed the Fenton reaction in a cell-free assay, but only DEP resulted in the generation of ROS as measured by dichlorodihydrofluorescein diacetate oxidation in mammalian cells. However, there was no evidence that increased ROS was a consequence of polycyclic aromatic hydrocarbon metabolism via CYP1A1 induction as urban dust, the Manchester dust samples but not DEP-induced CYP1A1 expression. Urban dust was more cytotoxic in murine embryonic fibroblasts (MEFs) than the other PM samples and also induced expression of GADD45a in the GreenScreen Human Cell assay without S9 activation suggesting the presence of a direct-acting genotoxicant. Urban dust and DEP produced comparable levels of DNA damage, as assessed by the alkaline comet assay, in MEFs at higher levels than those induced by Manchester PM. In conclusion, results from the cytotoxic and genotoxic assays are not consistent with ROS production being the sole determinant of PM-induced toxicity. This suggests that the organic component can contribute significantly to this toxicity and that further work is required to better characterise the extent to which ROS and organic components contribute to PM-induced toxicity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.