ArticleLab on a chip2015
Multiplexed detection of viral infections using rapid in situ RNA analysis on a chip.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Paired Capture and FISH Detection of Individual Virions Enable Cell-Free Determination of Infectious Titers.ACS sensors · 2023Article
- Illuminating RNA biology through imaging.Nature cell biology · 2022Review
- Recent Advances in Plasma-Engineered Polymers for Biomarker-Based Viral Detection and Highly Multiplexed Analysis.Biosensors · 2022Review
- Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH.Life science alliance · 2022Article
- Multiplexed detection of SARS-CoV-2 genomic and subgenomic RNA usingbioRxiv : the preprint server for biology · 2021Article
- Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2.Science advances · 2021Article
- Article
- Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies.Lab on a chip · 2020Article
- FISH and chips: a review of microfluidic platforms for FISH analysis.Medical microbiology and immunology · 2020Review
- smFISH in chips: a microfluidic-based pipeline to quantify in situ gene expression in whole organisms.Lab on a chip · 2020Article
- Mobile platform for rapid sub-picogram-per-milliliter, multiplexed, digital droplet detection of proteins.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Allele-specific RNA imaging shows that allelic imbalances can arise in tissues through transcriptional bursting.PLoS genetics · 2019Article
- A magnetic micropore chip for rapid (<1 hour) unbiased circulating tumor cell isolation and in situ RNA analysis.Lab on a chip · 2017Article
- Visualizing adenosine-to-inosine RNA editing in single mammalian cells.Nature methods · 2017Article
- Magnetic Nickel iron Electroformed Trap (MagNET): a master/replica fabrication strategy for ultra-high throughput (>100 mL h(-1)) immunomagnetic sorting.Lab on a chip · 2016Article
- Genome watch: The chronicles of virus-host affairs.Nature reviews. Microbiology · 2015Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Viral infections are a major cause of human disease, but many require molecular assays for conclusive diagnosis. Current assays typically rely on RT-PCR or ELISA; however, these tests often have limited speed, sensitivity or specificity. Here, we demonstrate that rapid RNA FISH is a viable alternative method that could improve upon these limitations. We describe a platform beginning with software to generate RNA FISH probes both for distinguishing related strains of virus (even those different by a single base) and for capturing large numbers of strains simultaneously. Next, we present a simple fluidic device for reliably performing RNA FISH assays in an automated fashion. Finally, we describe an automated image processing pipeline to robustly identify uninfected and infected samples. Together, our results establish RNA FISH as a methodology with potential for viral point-of-care diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.