ArticleBiophysical journal1989
Simultaneous imaging of cell and mitochondrial membrane potentials.
Article in Biophysical journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed.
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- Mitochondrial membrane potential and compartmentalized signaling: Calcium, ROS, and beyond.Redox biology · 2025Review
- Analysis of mitochondrial membrane potential, ROS, and calcium.Molecules and cells · 2025Article
- Acute myeloid leukemia mitochondria hydrolyze ATP to support oxidative metabolism and resist chemotherapy.Science advances · 2025Article
- Mitochondrial Morphofunctional Profiling in Primary Human Skin Fibroblasts Using TMRM and Mitotracker Green Co-staining.Methods in molecular biology (Clifton, N.J.) · 2025Article
- How and when to measure mitochondrial inner membrane potentials.Biophysical journal · 2024Review
- Intermittent proton bursts of single lactic acid bacteria.Chemical science · 2024Article
- Apurinic/apyrimidinic endonuclease 1 alleviates inflammation in fibroblast-like synoviocytes from patients with rheumatoid arthritis.Central-European journal of immunology · 2024Article
- Thioflavin T indicates mitochondrial membrane potential in mammalian cells.Biophysical reports · 2023Article
- The Use of Thioflavin T for the Estimation and Measurement of the Plasma Membrane Electric Potential Difference in Different Yeast Strains.Journal of fungi (Basel, Switzerland) · 2023Article
- Serotonin type-3 receptor antagonists selectively kill melanoma cells through classical apoptosis, microtubule depolymerisation, ERK activation, and NF-κB downregulation.Cell biology and toxicology · 2023Article
- Fluorescence microscopy imaging of mitochondrial metabolism in cancer cells.Frontiers in oncology · 2023Review
- Depolarized mitochondrial membrane potential and protection with duroquinone in isolated perfused lungs from rats exposed to hyperoxia.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Article
- Biomedical Applications of Translational Optical Imaging: From Molecules to Humans.Molecules (Basel, Switzerland) · 2021Review
- Defining the molecular mechanisms of the mitochondrial permeability transition through genetic manipulation of F-ATP synthase.Nature communications · 2021Article
- Flavonoids mitigate neurodegeneration in agedFood science & nutrition · 2020Article
- Activation of AMP-activated protein kinase during sepsis/inflammation improves survival by preserving cellular metabolic fitness.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
Abstract
The distribution of charged membrane-permeable molecular probes between intracellular organelles, the cytoplasm, and the outside medium is governed by the relative membrane electrical potentials of these regions through coupled equilibria described by the Nernst equation. A series of highly fluorescent cationic dyes of low membrane binding and toxicity (Ehrenberg, B., V. Montana, M.-D. Wei, J. P. Wuskell, and L. M. Loew, 1988. Biophys. J. 53:785-794) allows the monitoring of these equilibria through digital imaging video microscopy. We employ this combination of technologies to assess, simultaneously, the membrane potentials of cells and of their organelles in situ. We describe the methodology and optimal conditions for such measurements, and apply the technique to concomitantly follow, with good time resolution, the mitochondrial and plasma membrane potentials in several cultured cell lines. The time course of variations induced by chemical agents (ionophores, uncouplers, electron transport, and energy transfer inhibitors) in either or both these potentials is easily quantitated, and in accordance with mechanistic expectations. The methodology should therefore be applicable to the study of more subtle and specific, biologically induced potential changes in cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.