Evidence map›Paper›PMID 26113155›Full record

ArticleThe EMBO journal2015

Assembly of Slx4 signaling complexes behind DNA replication forks.

Attila Balint, TaeHyung Kim, David Gallo, Jose Renato Cussiol, Francisco M Bastos de Oliveira, Askar Yimit, Jiongwen Ou, Ryuichiro Nakato, Alexey Gurevich, Katsuhiko Shirahige and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The EMBO journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 45 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 3 countries.

Attila BalintDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada Donnelly Centre, University of Toronto, Toronto, ON, Canada.
TaeHyung KimDonnelly Centre, University of Toronto, Toronto, ON, Canada Department of Computer Science, University of Toronto, Toronto, ON, Canada.
David GalloDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada Donnelly Centre, University of Toronto, Toronto, ON, Canada.
Jose Renato CussiolDepartment of Molecular Biology and Genetics and Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA.
Francisco M Bastos de OliveiraDepartment of Molecular Biology and Genetics and Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA.
Askar YimitDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada Donnelly Centre, University of Toronto, Toronto, ON, Canada.
Jiongwen OuDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada Donnelly Centre, University of Toronto, Toronto, ON, Canada.
Ryuichiro NakatoInstitute of Molecular and Cellular Biosciences, Research Center for Epigenetic Disease, University of Tokyo, Tokyo, Japan.
Alexey GurevichDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada Donnelly Centre, University of Toronto, Toronto, ON, Canada.
Katsuhiko ShirahigeInstitute of Molecular and Cellular Biosciences, Research Center for Epigenetic Disease, University of Tokyo, Tokyo, Japan.
Marcus B SmolkaDepartment of Molecular Biology and Genetics and Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA.
Zhaolei ZhangDonnelly Centre, University of Toronto, Toronto, ON, Canada Department of Computer Science, University of Toronto, Toronto, ON, Canada.
Grant W BrownDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada Donnelly Centre, University of Toronto, Toronto, ON, Canada grant.brown@utoronto.ca.
University of Toronto · CACornell University · USThe University of Tokyo · JP

Funding

Cellular Responses to DNA Replication StressR01GM097272 · NIGMS · CORNELL UNIVERSITY · PI SMOLKA, MARCUS · 2011 to 2020
$2.7M
Canadian Institutes of Health Research MOP-79368NIGMS NIH HHS R01 GM097272NIGMS NIH HHS R01-GM097272
6 · The paper itself

Abstract

Obstructions to replication fork progression, referred to collectively as DNA replication stress, challenge genome stability. In Saccharomyces cerevisiae, cells lacking RTT107 or SLX4 show genome instability and sensitivity to DNA replication stress and are defective in the completion of DNA replication during recovery from replication stress. We demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. Slx4 complex formation is nucleated by Mec1 phosphorylation of histone H2A, which is recognized by the constitutive Slx4 binding partner Rtt107. Slx4 is essential for recruiting the Mec1 activator Dpb11 behind stressed replication forks, and Slx4 complexes are important for full activity of Mec1. We propose that Slx4 complexes promote robust checkpoint signaling by Mec1 by stably recruiting Dpb11 within a discrete domain behind the replication fork, during DNA replication stress.

Indexed as

DNA ReplicationProtein MultimerizationCell Cycle ProteinsDNA, FungalEndodeoxyribonucleasesHistonesIntracellular Signaling Peptides and ProteinsNuclear ProteinsProtein BindingProtein Serine-Threonine KinasesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsCell Cycle ProteinsDNA, FungalDPB11 protein, S cerevisiaeEndodeoxyribonucleasesHistonesIntracellular Signaling Peptides and ProteinsMEC1 protein, S cerevisiaeNuclear ProteinsProtein Serine-Threonine KinasesRTT107 protein, S cerevisiaeSaccharomyces cerevisiae ProteinsSLX4 protein, S cerevisiaecheckpoint kinaseDNA damage responseDpb11replication stressSlx4

Identifiers

PMID26113155
PMCPMC4557669
OpenAlexW2170295392

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.