ArticleBiochimica et biophysica acta2015
Diffuse, non-polar electropermeabilization and reduced propidium uptake distinguish the effect of nanosecond electric pulses.
Article in Biochimica et biophysica acta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Peculiarities of Phosphatidylserine Externalization by Nano- and Microsecond Electric Pulses.The Journal of membrane biology · 2026Article
- Nanosecond pulsed electric fields induce cell-size-dependent selective permeabilization of urothelial cancer cells.Communications biology · 2025Article
- Nanosecond pulsed electric fields increase antibiotic susceptibility in methicillin-resistantMicrobiology spectrum · 2024Article
- Genetically engineered HEK cells as a valuable tool for studying electroporation in excitable cells.Scientific reports · 2024Article
- Pulsed Field Ablation for the Treatment of Atrial Fibrillation: A Review and a Look into its Future.The heart surgery forum · 2024Article
- Surgical Ablation of Cardiac Tissue with Nanosecond Pulsed Electric Fields in Swine.Cardiovascular engineering and technology · 2023Article
- Electroporation and Electrochemotherapy in Gynecological and Breast Cancer Treatment.Molecules (Basel, Switzerland) · 2022Review
- Pulsed Field Ablation to Treat Atrial Fibrillation: A Review of the Literature.Journal of cardiovascular development and disease · 2022Review
- How to alleviate cardiac injury from electric shocks at the cellular level.Frontiers in cardiovascular medicine · 2022Article
- Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro.Molecules (Basel, Switzerland) · 2021Article
- Ablation Modalities for Therapeutic Intervention in Arrhythmia-Related Cardiovascular Disease: Focus on Electroporation.Journal of clinical medicine · 2021Review
- The interplay of excitation and electroporation in nanosecond pulse stimulation.Bioelectrochemistry (Amsterdam, Netherlands) · 2020Review
- Nanosecond pulsed electric fields modulate the expression of the astaxanthin biosynthesis genes psy, crtR-b and bkt 1 in Haematococcus pluvialis.Scientific reports · 2020Article
- Pulsed Electric Fields Can Create Pores in the Voltage Sensors of Voltage-Gated Ion Channels.Biophysical journal · 2020Article
- Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of CaInternational journal of molecular sciences · 2020Article
- Using Nanosecond Shocks for Cardiac Defibrillation.Bioelectricity · 2019Review
- Excitation of murine cardiac myocytes by nanosecond pulsed electric field.Journal of cardiovascular electrophysiology · 2019Article
- Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks.Scientific reports · 2018Article
- Asymmetric Patterns of Small Molecule Transport After Nanosecond and Microsecond Electropermeabilization.The Journal of membrane biology · 2018Article
- Damage-free peripheral nerve stimulation by 12-ns pulsed electric field.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Ca2+ activation and membrane electroporation by 10-ns and 4-ms electric pulses (nsEP and msEP) were compared in rat embryonic cardiomyocytes. The lowest electric field which triggered Ca2+ transients was expectedly higher for nsEP (36 kV/cm) than for msEP (0.09 kV/cm) but the respective doses were similar (190 and 460 mJ/g). At higher intensities, both stimuli triggered prolonged firing in quiescent cells. An increase of basal Ca2+ level by >10 nM in cells with blocked voltage-gated Ca2+ channels and depleted Ca2+ depot occurred at 63 kV/cm (nsEP) or 0.14 kV/cm (msEP) and was regarded as electroporation threshold. These electric field values were at 150-230% of stimulation thresholds for both msEP and nsEP, notwithstanding a 400,000-fold difference in pulse duration. For comparable levels of electroporative Ca2+ uptake, msEP caused at least 10-fold greater uptake of propidium than nsEP, suggesting increased yield of larger pores. Electroporation by msEP started Ca2+ entry abruptly and locally at the electrode-facing poles of cell, followed by a slow diffusion to the center. In a stark contrast, nsEP evoked a "supra-electroporation" pattern of slower but spatially uniform Ca2+ entry. Thus nsEP and msEP had comparable dose efficiency, but differed profoundly in the size and localization of electropores.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.