ArticleMicrobial biotechnology2015
Identification and characterization of a HEPN-MNT family type II toxin-antitoxin in Shewanella oneidensis.
Article in Microbial biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Phosphorothioate DNA modification by BREX type 4 systems in the human gut microbiome.Nature communications · 2026Article
- The Prevalence and Diversity of Marine Toxin-Antitoxin Systems.Marine drugs · 2025Article
- The VapBC-4 Characterization Indicates It Is a Bona Fide Toxin-Antitoxin Module ofMicroorganisms · 2025Article
- Structural insight into the distinct regulatory mechanism of the HEPN-MNT toxin-antitoxin system in Legionella pneumophila.Nature communications · 2024Article
- A nuclease domain fused to the Snf2 helicase confers antiphage defence in coral-associated Halomonas meridiana.Microbial biotechnology · 2024Article
- Focused Overview ofBiomimetics (Basel, Switzerland) · 2023Review
- Identification and Characterization of HEPN-MNT Type II TA System from Methanothermobacter thermautotrophicus ΔH.Journal of microbiology (Seoul, Korea) · 2023Article
- Toxin-antitoxin systems in bacterial pathogenesis.Heliyon · 2023Review
- The HipAB Toxin-Antitoxin System Stabilizes a Composite Genomic Island inFrontiers in microbiology · 2022Article
- Bacterial toxin-antitoxin modules: classification, functions, and association with persistence.Current research in microbial sciences · 2021Review
- Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA.Nucleic acids research · 2021Article
- HEPN RNases - an emerging class of functionally distinct RNA processing and degradation enzymes.Critical reviews in biochemistry and molecular biology · 2021Review
- An Auto-Regulating Type II Toxin-Antitoxin System Modulates Drug Resistance and Virulence inFrontiers in microbiology · 2021Article
- Novel polyadenylylation-dependent neutralization mechanism of the HEPN/MNT toxin/antitoxin system.Nucleic acids research · 2020Article
- Toxin-Antitoxin Gene Pairs Found in TnmBio · 2020Article
- The Variety in the Common Theme of Translation Inhibition by Type II Toxin-Antitoxin Systems.Frontiers in genetics · 2020Review
- Towards Exploring Toxin-Antitoxin Systems inInternational journal of molecular sciences · 2019Article
- Characterization and comparative analysis of toxin-antitoxin systems in Acetobacter pasteurianus.Journal of industrial microbiology & biotechnology · 2019Article
- Characterization of Two Toxin-Antitoxin Systems in Deep-SeaMarine drugs · 2019Article
- Structure-function analyses reveal the molecular architecture and neutralization mechanism of a bacterial HEPN-MNT toxin-antitoxin system.The Journal of biological chemistry · 2018Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxin-antitoxin (TA) systems are prevalent in bacteria and archaea. However, related studies in the ecologically and bioelectrochemically important strain Shewanella oneidensis are limited. Here, we show that SO_3166, a member of the higher eukaryotes and prokaryotes nucleotide-binding (HEPN) superfamily, strongly inhibited cell growth in S. oneidensis and Escherichia coli. SO_3165, a putative minimal nucleotidyltransferase (MNT), neutralized the toxicity of SO_3166. Gene SO_3165 lies upstream of SO_3166, and they are co-transcribed. Moreover, the SO_3165 and SO_3166 proteins interact with each other directly in vivo, and antitoxin SO_3165 bound to the promoter of the TA operon and repressed its activity. Finally, the conserved Rx4-6H domain in HEPN family was identified in SO_3166. Mutating either the R or H abolished SO_3166 toxicity, confirming that Rx4-6H domain is critical for SO_3166 activity. Taken together, these results demonstrate that SO_3166 and SO_3165 in S. oneidensis form a typical type II TA pair. This TA pair plays a critical role in regulating bacterial functions because its disruption led to impaired cell motility in S. oneidensis. Thus, we demonstrated for the first time that HEPN-MNT can function as a TA system, thereby providing important insights into the understanding of the function and regulation of HEPNs and MNTs in prokaryotes.
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