ArticleBMC evolutionary biology2015
Evolution of substrate recognition sites (SRSs) in cytochromes P450 from Apiaceae exemplified by the CYP71AJ subfamily.
Article in BMC evolutionary biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 52 citations in OpenAlex.
- Structure-based modeling reveals molecular basis for CYP153A6's novel activity toward toluene derivatives.Scientific reports · 2026Article
- Two types of coumarins-specific enzymes complete the last missing steps in pyran- and furanocoumarins biosynthesis.Acta pharmaceutica Sinica. B · 2024Article
- The telomere-to-telomere (T2T) genome of Peucedanum praeruptorum Dunn provides insights into the genome evolution and coumarin biosynthesis.GigaScience · 2024Article
- Status of Fungicide Resistance and Physiological Characterization of Tebuconazole Resistance inCurrent issues in molecular biology · 2022Article
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- Characterization of Cytochrome P450s with Key Roles in Determining Herbicide Selectivity in Maize.ACS omega · 2022Article
- First purified recombinant CYP75B including transmembrane helix with unexpected high substrate specificity to (2R)-naringenin.Scientific reports · 2022Article
- Cytochrome P450-catalyzed biosynthesis of furanoditerpenoids in the bioenergy crop switchgrass (Panicum virgatum L.).The Plant journal : for cell and molecular biology · 2021Article
- A cytochrome P450 CYP81AM1 from Tripterygium wilfordii catalyses the C-15 hydroxylation of dehydroabietic acid.Planta · 2021Article
- Impact of Genomic and Transcriptomic Resources on Apiaceae Crop Breeding Strategies.International journal of molecular sciences · 2021Review
- Two CYP71AJ enzymes function as psoralen synthase and angelicin synthase in the biosynthesis of furanocoumarins in Peucedanum praeruptorum Dunn.Plant molecular biology · 2020Article
- Angelicin-A Furocoumarin Compound With Vast Biological Potential.Frontiers in pharmacology · 2020Review
- Article
- Insights into Heterologous Biosynthesis of Arteannuin B and Artemisinin inMolecules (Basel, Switzerland) · 2019Article
- Engineering of CYP76AH15 can improve activity and specificity towards forskolin biosynthesis in yeast.Microbial cell factories · 2018Article
- The CYP71AZ P450 Subfamily: A Driving Factor for the Diversification of Coumarin Biosynthesis in Apiaceous Plants.Frontiers in plant science · 2018Article
- Localization and in-Vivo Characterization ofPlant physiology · 2017Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLarge proliferations of cytochrome P450 encoding genes resulting from gene duplications can be termed as 'blooms', providing genetic material for the genesis and evolution of biosynthetic pathways. Furanocoumarins are allelochemicals produced by many of the species in Apiaceaous plants belonging to the Apioideae subfamily of Apiaceae and have been described as being involved in the defence reaction against phytophageous insects.
resultsA bloom in the cytochromes P450 CYP71AJ subfamily has been identified, showing at least 2 clades and 6 subclades within the CYP71AJ subfamily. Two of the subclades were functionally assigned to the biosynthesis of furanocoumarins. Six substrate recognition sites (SRS1-6) important for the enzymatic conversion were investigated in the described cytochromes P450 and display significant variability within the CYP71AJ subfamily. Homology models underline a significant modification of the accession to the iron atom, which might explain the difference of the substrate specificity between the cytochromes P450 restricted to furanocoumarins as substrates and the orphan CYP71AJ.
conclusionTwo subclades functionally assigned to the biosynthesis of furanocoumarins and four other subclades were identified and shown to be part of two distinct clades within the CYP71AJ subfamily. The subclades show significant variability within their substrate recognition sites between the clades, suggesting different biochemical functions and providing insights into the evolution of cytochrome P450 'blooms' in response to environmental pressures.
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