ArticleJournal of the American Society for Mass Spectrometry2015
A calibration routine for efficient ETD in large-scale proteomics.
Article in Journal of the American Society for Mass Spectrometry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Deciphering cytokine-driven ADP-ribosylation signaling networks via Af1521-based mass spectrometry analysis of labile Glu/Asp-linkages.Nature communications · 2026Article
- The protease cathepsin K can debulk the cancer glycocalyx.The Journal of biological chemistry · 2026Article
- Improvements in Glycoproteomics through Architecture Changes to the Orbitrap Tribrid MS Platform.Analytical chemistry · 2025Article
- Autonomous Dissociation-type Selection for Glycoproteomics Using a Real-Time Library Search.Journal of proteome research · 2024Article
- Custom Workflow for the Confident Identification of Sulfotyrosine-Containing Peptides and Their Discrimination from Phosphopeptides.Journal of proteome research · 2023Article
- Discovery and characterization of a new class of O-linking oligosaccharyltransferases from the Moraxellaceae family.Glycobiology · 2023Article
- DecipheringMolecular omics · 2022Article
- Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences.The Journal of biological chemistry · 2022Article
- Practical Effects of Intramolecular Hydrogen Rearrangement in Electron Transfer Dissociation-Based Proteomics.Journal of the American Society for Mass Spectrometry · 2022Article
- Maximizing Depth of PTM Coverage: Generating Robust MS Datasets for Computational Prediction Modeling.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Improved Sequence Analysis of Intact Proteins by Parallel Ion Parking during Electron Transfer Dissociation.Analytical chemistry · 2021Article
- The regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome.Nature communications · 2021Article
- LYTACs that engage the asialoglycoprotein receptor for targeted protein degradation.Nature chemical biology · 2021Article
- Analysis of Bovine Kappa-Casein Glycomacropeptide by Liquid Chromatography-Tandem Mass Spectrometry.Foods (Basel, Switzerland) · 2021Article
- Electron-Based Dissociation Is Needed for O-Glycopeptides Derived from OpeRATOR Proteolysis.Analytical chemistry · 2020Article
- Mapping Physiological ADP-Ribosylation Using Activated Ion Electron Transfer Dissociation.Cell reports · 2020Article
- Optical Fiber-Enabled Photoactivation of Peptides and Proteins.Analytical chemistry · 2020Article
- A Perspective on the Confident Comparison of Glycoprotein Site-Specific Glycosylation in Sample Cohorts.Biochemistry · 2020Review
- Optimal Dissociation Methods Differ forJournal of proteome research · 2020Article
- Top-Down Characterization of an Intact Monoclonal Antibody Using Activated Ion Electron Transfer Dissociation.Analytical chemistry · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Electron transfer dissociation (ETD) has been broadly adopted and is now available on a variety of commercial mass spectrometers. Unlike collisional activation techniques, optimal performance of ETD requires considerable user knowledge and input. ETD reaction duration is one key parameter that can greatly influence spectral quality and overall experiment outcome. We describe a calibration routine that determines the correct number of reagent anions necessary to reach a defined ETD reaction rate. Implementation of this automated calibration routine on two hybrid Orbitrap platforms illustrate considerable advantages, namely, increased product ion yield with concomitant reduction in scan rates netting up to 75% more unique peptide identifications in a shotgun experiment. Graphical Abstract ᅟ.
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