ArticlePloS one2015
Guanidinoacetate is more effective than creatine at enhancing tissue creatine stores while consequently limiting methionine availability in Yucatan miniature pigs.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- Guanidinoacetic acid enhances Tibetan sheep-meat quality through the gut-muscle axis: insights from metabolome-microbiome integration.Food chemistry. Molecular sciences · 2026Article
- Growth Characteristics, Blood Biochemistry, Histology, and Metabolic Profile of Muscle and Different Tissues: Toxicity Study of Deoxynivalenol.Food science of animal resources · 2025Article
- Guanidinoacetic acid regulated postmortem muscle glycolysis associated with AMPK signaling and protein acetylation.Animal bioscience · 2025Article
- Feeding Parenteral Nutrition in the Neonatal Period Programs Dyslipidemia in Adulthood in Yucatan Miniature Pigs.The Journal of nutrition · 2024Article
- Effects of Dietary Guanidinoacetic Acid on the Performance, Rumen Fermentation, Metabolism, and Meat of Confined Steers.Animals : an open access journal from MDPI · 2024Article
- Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation.eLife · 2024Article
- Effects of guanidine acetic acid supplementation from gestation to lactation on reproductive performance, colostrum quality, blood biochemistry, and intestinal microflora diversity of sows.Frontiers in veterinary science · 2024Article
- Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation.bioRxiv : the preprint server for biology · 2023Article
- Molecular Mechanism Biomarkers Predict Diagnosis in Schizophrenia and Schizoaffective Psychosis, with Implications for Treatment.International journal of molecular sciences · 2023Article
- Molecular Mechanisms Provide a Landscape for Biomarker Selection for Schizophrenia and Schizoaffective Psychosis.International journal of molecular sciences · 2023Review
- Dietary creatine and guanidinoacetic acid supplementation have limited effects on hybrid striped bass.Fish physiology and biochemistry · 2023Article
- Guanidine Acetic Acid Alters Tissue Bound Amino Acid Profiles and Oxidative Status in Finishing Pigs.Animals : an open access journal from MDPI · 2023Article
- Dietary guanidinoacetic acid supplementation improves water holding capacity and lowers free amino acid concentration of fresh meat in finishing pigs fed with various dietary protein levels.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Effect of guanidinoacetic acid supplementation on nitrogen retention and methionine methyl group flux in growing steers fed corn-based diets.Journal of animal science · 2022Article
- Guanidine acetic acid supplementation altered plasma and tissue free amino acid profiles in finishing pigs.Porcine health management · 2022Article
- Review
- Effect of guanidine acetic acid on meat quality, muscle amino acids, and fatty acids in Tibetan pigs.Frontiers in veterinary science · 2022Article
- Guanidinoacetic Acid as a Nutritional Adjuvant to Multiple Sclerosis Therapy.Frontiers in human neuroscience · 2022Review
- Addition of a combination of creatine, carnitine, and choline to a commercial diet increases postprandial plasma creatine and creatinine concentrations in adult dogs.Frontiers in veterinary science · 2022Article
- Addition of dietary methionine but not dietary taurine or methyl donors/receivers to a grain-free diet increases postprandial homocysteine concentrations in adult dogs.Journal of animal science · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Creatine (Cr) is an important high-energy phosphate buffer in tissues with a high energy demand such as muscle and brain and is consequently a highly consumed nutritional supplement. Creatine is synthesized via the S-adenosylmethionine (SAM) dependent methylation of guanidinoacetate (GAA) which is not regulated by a feedback mechanism. The first objective of this study was to determine the effectiveness of GAA at increasing tissue Cr stores. Because SAM is required for other methylation reactions, we also wanted to determine whether an increased creatine synthesis would lead to a lower availability of methyl groups for other methylated products. Three month-old pigs (n = 18) were fed control, GAA- or Cr-supplemented diets twice daily. On day 18 or 19, anesthesia was induced 1-3 hours post feeding and a bolus of [methyl-3H]methionine was intravenously infused. After 30 minutes, the liver was analyzed for methyl-3H incorporation into protein, Cr, phosphatidylcholine (PC) and DNA. Although both Cr and GAA led to higher hepatic Cr concentration, only supplementation with GAA led to higher levels of muscle Cr (P < 0.05). Only GAA supplementation resulted in lower methyl-3H incorporation into PC and protein as well as lower hepatic SAM concentration compared to the controls, suggesting that Cr synthesis resulted in a limited methyl supply for PC and protein synthesis (P < 0.05). Although GAA is more effective than Cr at supporting muscle Cr accretion, further research should be conducted into the long term consequences of a limited methyl supply and its effects on protein and PC homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.