ArticlePloS one2015
Development of a Bioluminescent Nitroreductase Probe for Preclinical Imaging.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- A genetic reporter for visualizing nitroreductase activity using magnetic resonance imaging.Chemistry methods : new approaches to solving problems in chemistry · 2026Article
- Fetal programming by the parental microbiome of offspring behavior, and DNA methylation and gene expression within the hippocampus.Microbiome · 2025Article
- Hydrolysis-Resistant Ester-Based Linkers for Development of Activity-Based NIR Bioluminescence Probes.Journal of the American Chemical Society · 2023Article
- Activity-Based NIR Bioluminescence Probe Enables Discovery of Diet-Induced Modulation of the Tumor Microenvironment via Nitric Oxide.ACS central science · 2022Article
- Portable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals.Nature communications · 2021Article
- Molecular Design of d-Luciferin-Based Bioluminescence and 1,2-Dioxetane-Based Chemiluminescence Substrates for Altered Output Wavelength and Detecting Various Molecules.Molecules (Basel, Switzerland) · 2021Review
- Targeted Delivery of Persulfides to the Gut: Effects on the Microbiome.Angewandte Chemie (International ed. in English) · 2021Article
- RepurposingTheranostics · 2021Article
- Positron Emission Tomography Imaging ofACS infectious diseases · 2020Article
- Rapid differentiation between bacterial infections and cancer using a near-infrared fluorogenic probe.Chemical science · 2020Article
- Caged luciferins for bioluminescent activity-based sensing.Current opinion in biotechnology · 2019Review
- Recent progress in the imaging detection of enzyme activitiesRSC advances · 2019Review
- Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- In vivo bioluminescence imaging of labile iron accumulation in a murine model ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Probe for the Detection of Hypoxic Cancer Cells.ACS sensors · 2017Article
- Development of a Click Beetle Luciferase Reporter System for Enhanced Bioluminescence Imaging ofFrontiers in microbiology · 2017Article
- In Vivo Chemiluminescent Imaging Agents for Nitroreductase and Tissue Oxygenation.Analytical chemistry · 2016Article
- The Expanding Toolbox of In Vivo Bioluminescent Imaging.Frontiers in oncology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial nitroreductases (NTRs) have been widely utilized in the development of novel antibiotics, degradation of pollutants, and gene-directed enzyme prodrug therapy (GDEPT) of cancer that reached clinical trials. In case of GDEPT, since NTR is not naturally present in mammalian cells, the prodrug is activated selectively in NTR-transformed cancer cells, allowing high efficiency treatment of tumors. Currently, no bioluminescent probes exist for sensitive, non-invasive imaging of NTR expression. We therefore developed a "NTR caged luciferin" (NCL) probe that is selectively reduced by NTR, producing light proportional to the NTR activity. Here we report successful application of this probe for imaging of NTR in vitro, in bacteria and cancer cells, as well as in vivo in mouse models of bacterial infection and NTR-expressing tumor xenografts. This novel tool should significantly accelerate the development of cancer therapy approaches based on GDEPT and other fields where NTR expression is important.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.