ArticlePloS one2015
Evaluation of Appropriate Reference Genes for Gene Expression Normalization during Watermelon Fruit Development.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Selection and verification of reliable internal reference genes in stem development of herbaceous peony (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Thioredoxin h2 inhibits the MPKK5-MPK3 cascade to regulate the CBF-COR signaling pathway inHorticulture research · 2023Article
- The branchless gene Clbl in watermelon encoding a TERMINAL FLOWER 1 protein regulates the number of lateral branches.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Genome-wide analysis of IQD proteins and ectopic expression of watermelon ClIQD24 in tomato suggests its important role in regulating fruit shape.Frontiers in genetics · 2022Article
- Transcriptome regulation of carotenoids in five flesh-colored watermelons (Citrullus lanatus).BMC plant biology · 2021Article
- Resistant Sources and Genetic Control of Resistance to ToLCNDV in Cucumber.Microorganisms · 2021Article
- Comprehensive evaluation of candidate reference genes for quantitative real-time PCR-based analysis in Caucasian clover.Scientific reports · 2021Article
- Selection of Suitable Reference Genes for qPCR Gene Expression Analysis of HepG2 and L02 in Four Different Liver Cell Injured Models.BioMed research international · 2020Article
- A cucumber green mottle mosaic virus vector for virus-induced gene silencing in cucurbit plants.Plant methods · 2020Article
- Article
- Genetic mapping and development of molecular markers for a candidate gene locus controlling rind color in watermelon.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Selection of reliable reference genes for quantitative RT-PCR in garlic under salt stress.PeerJ · 2019Article
- Selection of the optimal reference genes for expression analyses in different materials ofPlant methods · 2019Article
- Genetic Mapping and Discovery of the Candidate Gene for Black Seed Coat Color in Watermelon (Frontiers in plant science · 2019Article
- Genetic mapping reveals a candidate gene (ClFS1) for fruit shape in watermelon (Citrullus lanatus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Article
- Genetic mapping reveals a marker for yellow skin in watermelon (Citrullus lanatus L.).PloS one · 2018Article
- Comprehensive evaluation of blood-brain barrier-forming micro-vasculatures: Reference and marker genes with cellular composition.PloS one · 2018Article
- Genome-wide identification of new reference genes for RT-qPCR normalization in CGMMV-infectedPeerJ · 2018Article
- Genome-Wide Identification and Evaluation of Reference Genes for Quantitative RT-PCR Analysis during Tomato Fruit Development.Frontiers in plant science · 2017Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Gene expression analysis in watermelon (Citrullus lanatus) fruit has drawn considerable attention with the availability of genome sequences to understand the regulatory mechanism of fruit development and to improve its quality. Real-time quantitative reverse-transcription PCR (qRT-PCR) is a routine technique for gene expression analysis. However, appropriate reference genes for transcript normalization in watermelon fruits have not been well characterized. The aim of this study was to evaluate the appropriateness of 12 genes for their potential use as reference genes in watermelon fruits. Expression variations of these genes were measured in 48 samples obtained from 12 successive developmental stages of parthenocarpic and fertilized fruits of two watermelon genotypes by using qRT-PCR analysis. Considering the effects of genotype, fruit setting method, and developmental stage, geNorm determined clathrin adaptor complex subunit (ClCAC), β-actin (ClACT), and alpha tubulin 5 (ClTUA5) as the multiple reference genes in watermelon fruit. Furthermore, ClCAC alone or together with SAND family protein (ClSAND) was ranked as the single or two best reference genes by NormFinder. By using the top-ranked reference genes to normalize the transcript abundance of phytoene synthase (ClPSY1), a good correlation between lycopene accumulation and ClPSY1 expression pattern was observed in ripening watermelon fruit. These validated reference genes will facilitate the accurate measurement of gene expression in the studies on watermelon fruit biology.
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