ArticleThe EPMA journal2015
The role of beneficial bacteria wall elasticity in regulating innate immune response.
Article in The EPMA journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
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- Overview of the immunomodulatory role of bacterial probiotic-derived peptidoglycan: from molecular insights to therapeutic application.Frontiers in microbiology · 2026Review
- Impact of a 7-day short peptide diet on gut microbiota and metabolomics in septic mice.Frontiers in nutrition · 2025Article
- Bacterium-like Particles fromVaccines · 2024Article
- Probiotics therapy show significant improvement in obesity and neurobehavioral disorders symptoms.Frontiers in cellular and infection microbiology · 2023Review
- On the Determination of Mechanical Properties of Aqueous Microgels-Towards High-Throughput Characterization.Gels (Basel, Switzerland) · 2021Article
- Probiotics in Cosmetic and Personal Care Products: Trends and Challenges.Molecules (Basel, Switzerland) · 2021Review
- Intestinal alkaline phosphatase modulation by food components: predictive, preventive, and personalized strategies for novel treatment options in chronic kidney disease.The EPMA journal · 2020Review
- Variations in the Morphology, Mechanics and Adhesion of Persister and ResisterAntibiotics (Basel, Switzerland) · 2020Article
- Effect of probiotics on obesity-related markers per enterotype: a double-blind, placebo-controlled, randomized clinical trial.The EPMA journal · 2020Article
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- The Impact of Diet on the Involvement of Non-Coding RNAs, Extracellular Vesicles, and Gut Microbiome-Virome in Colorectal Cancer Initiation and Progression.Frontiers in oncology · 2020Review
- ProbioticThe EPMA journal · 2019Article
- Changes in Cellular Elasticities and Conformational Properties of Bacterial Surface Biopolymers of Multidrug-ResistantCell surface (Amsterdam, Netherlands) · 2019Article
- Can tailored nanoceria act as a prebiotic? Report on improved lipid profile and gut microbiota in obese mice.The EPMA journal · 2019Article
- Specific properties of probiotic strains: relevance and benefits for the host.The EPMA journal · 2018Article
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- Altered Gut Microbiota Composition in Subjects Infected WithFrontiers in microbiology · 2018Article
- Comparative study of probiotic effects ofThe EPMA journal · 2017Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProbiotics have great potential to contribute to development of healthy dietary regimes, preventive care, and an integrated approach to immunity-related disease management. The bacterial wall is a dynamic entity, depending on many components and playing an essential role in modulating immune response. The impact of cell wall elasticity on the beneficial effects of probiotic strains has not been sufficiently studied. The aim was to investigate the effect of lactic acid bacteria (LAB) and bifidobacteria strains on phagocytic system cells (macrophages) as related to bacterial wall elasticity, estimated using atomic force microscopy (AFM).
methodsWe conducted studies on Balb/c line mice 18-20 g in weight using lyophilized strains of LAB-Lactobacillus acidophilus IMV B-7279, Lactobacillus casei IMV B-7280, Lactobacillus delbrueckii subsp. bulgaricus IMV B-7281, and bifidobacteria-Bifidobacterium animalis VKL and Bifidobacterium animalis VKB. We cultivated the macrophages obtained from the peritoneal cavity of mice individually with the strains of LAB and bifidobacteria and evaluated their effect on macrophages, oxygen-dependent bactericidal activity, nitric oxide production, and immunoregulatory cytokines. We used AFM scanning to estimate bacterial cell wall elasticity.
resultsAll strains had a stimulating effect on the functional activity of macrophages and ability to produce NO/NO2 in vitro. Lactobacilli strains increased the production of IL-12 and IFN-γ in vitro. The AFM demonstrated different cell wall elasticity levels in various strains of LAB and bifidobacteria. The rigidity of the cell walls among lactobacilli was distributed as follows: Lactobacillus acidophilus IMV B-7279 > Lactobacillus casei IMV B-7280 > Lactobacillus delbrueckii subsp. bulgaricus IMV B-7281; among the strains of bifidobacteria: B. animalis VKB > B. animalis VKL. Probiotic strain survival in the macrophages depended on the bacterial cell wall elasticity and on the time of their joint cultivation.
conclusionLAB and bifidobacteria strains stimulate immune-modulatory cytokines and active oxygen and nitrogen oxide compound production in macrophages. Strains with a more elastic cell wall according to AFM data demonstrated higher resistance to intracellular digestion in macrophages and higher level of their activation. AFM might be considered as a fast and accurate method to assess parameters of probiotic strain cell wall to predict their immune-modulatory properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.