Trial reportBiochemia medica2015
Method evaluation study of a new generation of vitamin D assays.
Trial report in Biochemia medica, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Efficacy and safety of vitamin D supplementation in treating pulmonary tuberculosis: a systematic review and meta-analysis.BMC infectious diseases · 2026Pooled it
- A randomized controlled trial of vitamin D supplementation on perinatal depression: in Iranian pregnant mothers.BMC pregnancy and childbirth · 2016Trial
- Evaluation of the Performance of New Fluorescence Immunoassay POCTs for Determining the Value of Vitamin D in Whole Blood.Molecules (Basel, Switzerland) · 2025Article
- Comparison of the vitamin D level between children with and without cow's milk protein allergy: a systematic review with meta-analysis.Frontiers in pediatrics · 2025Article
- Serum 25-Hydroxyvitamin D Levels and Disease Activity in Patients with Systemic Lupus Erythematosus: An Exploratory Study in Western Mexico.Diseases (Basel, Switzerland) · 2024Article
- Label-Free Metal-Oxide Transistor Biosensors for Metabolite Detection in Human Saliva.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Rapid Determination of Vitamin DNanomaterials (Basel, Switzerland) · 2022Article
- Hormonal and metabolic profiles of obese and nonobese type 2 diabetes patients: implications of plasma insulin, ghrelin, and vitamin D levels.Cardiovascular endocrinology & metabolism · 2022Article
- Influence of Nordic Walking Training on Vitamin D Level in the Blood and Quality of Life among Women Aged 65-74.Healthcare (Basel, Switzerland) · 2021Article
- Comparative study of two immunoassays used for the determination of serum vitamin D.Practical laboratory medicine · 2021Article
- Musculoskeletal Health in Active Ambulatory Men with Cerebral Palsy and the Impact of Vitamin D.Nutrients · 2021Article
- Article
- The Effects of a Single Freeze-Thaw Cycle on Concentrations of Nutritional, Noncommunicable Disease, and Inflammatory Biomarkers in Serum Samples.Journal of laboratory physicians · 2021Article
- Immunomodulatory Function of Vitamin D and Its Role in Autoimmune Thyroid Disease.Frontiers in immunology · 2021Review
- The Effect of Vitamin DNutrients · 2020Article
- A Comparison between Three Different Automated Total 25-hydroxyvitamin D Immunoassay Methods and Ultra Performance Liquid Chromatography.Asian Pacific journal of cancer prevention : APJCP · 2020Article
- Quantitation of serum 25(OH)D2 and 25(OH)D3 concentrations by liquid chromatography tandem mass spectrometry in patients with diabetes mellitus.Journal of food and drug analysis · 2019Article
- Clinical Features of Psoriatic Arthritis: a Comprehensive Review of Unmet Clinical Needs.Clinical reviews in allergy & immunology · 2018Review
- Ocular Pharmacokinetics of 25-Hydroxyvitamin D3 After Weekly Supplementation in Rabbits Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometer.European journal of drug metabolism and pharmacokinetics · 2018Article
- Association between Fasting Glucose Concentration, Lipid Profile and 25(OH)D Status in Children Aged 9⁻11.Nutrients · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionRecently several diagnostic manufacturers have launched new 25-hydroxy-vitamin D (25[OH]D) assays, which are aligned to the National Institute of Standards and Technology (NIST) Standard Reference Materials (SRM) (NIST, Gaithersburg, Maryland). The aim of this study was to compare the performance of one liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, one enzyme linked immunosorbent assay (ELISA), and one recalibrated and previous version of a chemiluminescence immunoassay (CLIA). MATERIAL AND
methodsSerum-aliquots of 198 patient samples from routine 25(OH)D analysis were measured by the ClinMass® LC-MS/MS Complete Kit (RECIPE Chemicals+Instruments GmbH, Munich, Germany), the ORGENTEC 25(OH)D3/D2 ELISA (ORGENTEC Diagnostika GmbH, Mainz, Germany), the recalibrated Immunodiagnostic Systems (IDS)-iSYS 25(OH)DS and the previous used IDS-iSYS 25(OH)D CLIA (Immunodiagnostic Systems Ltd, Boldon, United Kingdom). Bland-Altman and Deming regression analyses were calculated for methods comparison of all tested 25(OH)D assays. The LC-MS/MS method was defined as the reference method. Within-run and between-run precision measurements were performed for all methods with three different concentration levels.
resultsCompared to the LC-MS/MS method, the new IDS-iSYS 25(OH)DS and ORGENTEC 25(OH)D3/D2 assay demonstrated mean relative biases of 16.3% and 17.8%. The IDS-iSYS 25(OH)D assay showed the lowest mean bias of 1.5%. Deming regression analyses of the recalibrated IDS-iSYS 25(OH)DS and the ORGENTEC 25(OH)D3/D2 assay showed proportional differences, when compared to the reference method. All assays showed a within-run and between-run imprecision of ≤20% at each of the evaluated concentration levels.
conclusionsThe evaluated standardized immunoassays and LC-MS/MS are useful methods for measuring 25(OH)D serum-levels in clinical laboratories.
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