ArticleBiotechnology for biofuels2015
Metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by Corynebacterium glutamicum.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 95 citations in OpenAlex.
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- Acetol biosynthesis enables NADPH balance during nitrogen limitation in engineered Escherichia coli.Microbial cell factories · 2025Article
- Isolation and characterization ofFrontiers in microbiology · 2025Article
- A genome-reduced Corynebacterium glutamicum derivative discloses a hidden pathway relevant for 1,2-propanediol production.Microbial cell factories · 2024Article
- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- Biosynthesis pathways of expanding carbon chains for producing advanced biofuels.Biotechnology for biofuels and bioproducts · 2023Review
- Metabolomics-Driven Identification of the Rate-Limiting Steps in 1-Propanol Production.Frontiers in microbiology · 2022Article
- Production of l-glutamate family amino acids inSynthetic and systems biotechnology · 2021Review
- Evolutionary Aspects of the Oxido-Reductive Network of Methylglyoxal.Journal of molecular evolution · 2021Article
- A comprehensive review on microbial production of 1,2-propanediol: micro-organisms, metabolic pathways, and metabolic engineering.Biotechnology for biofuels · 2021Review
- Induction of glutamic acid production by copper in Corynebacterium glutamicum.Applied microbiology and biotechnology · 2021Article
- Genome-scale insights into the metabolic versatility of Limosilactobacillus reuteri.BMC biotechnology · 2021Article
- Redirection of the central metabolism of Klebsiella pneumoniae towards dihydroxyacetone production.Microbial cell factories · 2021Article
- Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum.AMB Express · 2021Article
- Coenzyme QFrontiers in bioengineering and biotechnology · 2021Article
- A Timed Off-Switch for Dynamic Control of Gene Expression inFrontiers in bioengineering and biotechnology · 2021Article
- Engineering Escherichia coli for Direct Production of 1,2-Propanediol and 1,3-Propanediol from Starch.Current microbiology · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProduction of the versatile bulk chemical 1,2-propanediol and the potential biofuel 1-propanol is still dependent on petroleum, but some approaches to establish bio-based production from renewable feed stocks and to avoid toxic intermediates have been described. The biotechnological workhorse Corynebacterium glutamicum has also been shown to be able to overproduce 1,2-propanediol by metabolic engineering. Additionally, C. glutamicum has previously been engineered for production of the biofuels ethanol and isobutanol but not for 1-propanol.
resultsIn this study, the improved production of 1,2-propanediol by C. glutamicum is presented. The product yield of a C. glutamicum strain expressing the heterologous genes gldA and mgsA from Escherichia coli that encode methylglyoxal synthase gene and glycerol dehydrogenase, respectively, was improved by additional expression of alcohol dehydrogenase gene yqhD from E. coli leading to a yield of 0.131 mol/mol glucose. Deletion of the endogenous genes hdpA and ldh encoding dihydroxyacetone phosphate phosphatase and lactate dehydrogenase, respectively, prevented formation of glycerol and lactate as by-products and improved the yield to 0.343 mol/mol glucose. To construct a 1-propanol producer, the operon ppdABC from Klebsiella oxytoca encoding diol dehydratase was expressed in the improved 1,2-propanediol producing strain ending up with 12 mM 1-propanol and up to 60 mM unconverted 1,2-propanediol. Thus, B12-dependent diol dehydratase activity may be limiting 1-propanol production.
conclusionsProduction of 1,2-propanediol by C. glutamicum was improved by metabolic engineering targeting endogenous enzymes. Furthermore, to the best of our knowledge, production of 1-propanol by recombinant C. glutamicum was demonstrated for the first time.
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