ArticleJournal of biological engineering2015
Chemical lysis of cyanobacteria.
Article in Journal of biological engineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- Deciphering the metabolic details of L-lysine toxicity in cyanobacteria.Plant physiology · 2026Article
- Secretins of type-two secretion systems are necessary for exopolymeric slime secretion in cyanobacteria and myxobacteria.Nature communications · 2025Article
- Whole-cell hydrolysate of cyanobacteria enhances biofuel fermentation of plant biomass.Sustainable microbiology · 2025Article
- Article
- Article
- Cell Membrane Biomimetic Nano-Delivery Systems for Cancer Therapy.Pharmaceutics · 2023Review
- Cyanobacterial Algal Bloom Monitoring: Molecular Methods and Technologies for Freshwater Ecosystems.Microorganisms · 2023Review
- Sandwich Hybridization Assay for In Situ Real-Time Cyanobacterial Detection and Monitoring: A Review.Biosensors · 2022Review
- Comparative Assessment of Physical and Chemical Cyanobacteria Cell Lysis Methods for Total Microcystin-LR Analysis.Toxins · 2021Article
- Review of Microfluidic Methods for Cellular Lysis.Micromachines · 2021Review
- Novel perspective on a conventional technique: Impact of ultra-low temperature on bacterial viability and protein extraction.PloS one · 2021Article
- Methodologies for preparation of prokaryotic extracts for cell-free expression systems.Synthetic and systems biotechnology · 2020Review
- Optimization of the Freezing-Thawing Method for Extracting Phycobiliproteins fromMolecules (Basel, Switzerland) · 2020Article
- A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets.Journal of animal physiology and animal nutrition · 2020Article
- The Development of an Effective Bacterial Single-Cell Lysis Method Suitable for Whole Genome Amplification in Microfluidic Platforms.Micromachines · 2018Article
- Homeostasis of Second Messenger Cyclic-di-AMP Is Critical for Cyanobacterial Fitness and Acclimation to Abiotic Stress.Frontiers in microbiology · 2018Article
- Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a mixture of enzymes and chemicals that completely lyse cyanobacteria. Since the treatment involves only readily-available chemicals and simple proteins that degrade the components of the cyanobacterial cell wall, it can easily be used in high-throughput applications requiring lysis for subsequent intracellular measurements. Our lysis technique consistently enables complete lysis of several different cyanobacterial strains, and we demonstrated that DNA, mRNA, and proteins are preserved in the lysates. Chemical lysis can be superior to existing techniques because of its convenience, reliability, and amenability to a variety of downstream applications.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.