ArticleAllergy2015
Intrinsic properties of germinal center-derived B cells promote their enhanced class switching to IgE.
Article in Allergy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Tonsillar expression quantitative trait loci verify and expand genetic contributors to childhood atopic diseases.bioRxiv : the preprint server for biology · 2026Article
- Inhibition of PI3K p110δ activity reduces IgE production in IL-4 and anti-CD40 stimulated human B cell cultures.Immunology · 2023Article
- IgE+ plasmablasts predict the onset of clinical allergy.Frontiers in immunology · 2023Article
- Early IgE Production Is Linked with Extrafollicular B- and T-Cell Activation in Low-Dose Allergy Model.Vaccines · 2022Article
- IgE-expressing long-lived plasma cells in persistent sensitization.Frontiers in pediatrics · 2022Review
- Non-classical B Cell Memory of Allergic IgE Responses.Frontiers in immunology · 2019Review
- Transcriptional Analysis of the Human IgE-Expressing Plasma Cell Differentiation Pathway.Frontiers in immunology · 2019Article
- IgE Test in Secretions of Patients with Respiratory Allergy.Current allergy and asthma reports · 2018Review
- IgE repertoire and immunological memory: compartmental regulation and antibody function.International immunology · 2018Review
- Gut Mucosal Antibody Responses and Implications for Food Allergy.Frontiers in immunology · 2018Review
- Article
- Global gene regulation during activation of immunoglobulin class switching in human B cells.Scientific reports · 2016Article
- Intrinsic properties of germinal center-derived B cells promote their enhanced class switching to IgE.Allergy · 2015Article
- Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
backgroundResearch on the origins and development of human IgE-expressing (IgE(+) ) cells is required for understanding the pathogenesis of allergy and asthma. These studies have been thwarted by the rarity of IgE(+) cells in vivo and the low frequency of class switch recombination (CSR) to IgE ex vivo. To determine the main source of IgE(+) cells, we investigated the relation between the phenotypic composition of tonsil B cells and the CSR to IgE ex vivo.
methodsHuman tonsil B cells were analyzed by flow cytometry (FACS) and cultured with IL-4 and anti-CD40 to induce CSR to IgE. Naïve, germinal center (GC), early GC (eGC), and memory tonsil B cells were isolated by FACS, and their capacities for IL-4 and anti-CD40 signaling, cell proliferation, and de novo class switching to IgE were analyzed by RT-PCR and FACS.
resultsB cells from different tonsils exhibited varying capacities for CSR to IgE ex vivo. This was correlated with the percentage of eGC B cells in the tonsil at the outset of the culture. Despite relatively poor cell viability, eGC and GC B-cell cultures produced the highest yields of IgE(+) cells compared to naïve and memory B-cell cultures. The main factors accounting for this result were the strength of IL-4R and CD40 signaling and relative rates of cell proliferation.
conclusionsThis study shows that the maturation state of tonsil B cells determines their capacity to undergo class switching to IgE ex vivo, with the GC-derived B cells yielding the highest percentage of IgE(+) cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.