ArticleReproductive biology and endocrinology : RB&E2015
Vitrification of human ovarian tissue: a practical and relevant alternative to slow freezing.
Article in Reproductive biology and endocrinology : RB&E, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 3 of them syntheses that pooled it.
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Who cites it
26 citing papers in PubMed, 3 syntheses or guidelines pooled it, 61 citations in OpenAlex.
- Vitrification versus slow freezing of human ovarian tissue: a systematic review and meta-analysis of histological outcomes.Journal of assisted reproduction and genetics · 2023Pooled it
- Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis.Scientific reports · 2017Pooled it
- Recent advances in the field of ovarian tissue cryopreservation and opportunities for research.Journal of assisted reproduction and genetics · 2017Pooled it
- Transcriptomic insights into ovine ovarian response to extended in vitro culture and cryopreservation.Journal of assisted reproduction and genetics · 2026Article
- Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing.JBRA assisted reproduction · 2025Article
- Comparison of survival and apoptosis parameters of ovarian tissue follicles in two vitrification methods of needle-immersion and cryo-support in cancer patients.Turkish journal of obstetrics and gynecology · 2025Article
- The first comparative study on the effectiveness of slow freezing and vitrification of human ovarian tissue by xenotransplantation model in Vietnam.Journal of assisted reproduction and genetics · 2025Article
- Investigating the impact of vitrification on bovine ovarian tissue morphology, follicle survival, and transcriptomic signature.Journal of assisted reproduction and genetics · 2024Article
- Comparative effects of a calcium chelator (BAPTA-AM) and melatonin on cryopreservation-induced oxidative stress and damage in ovarian tissue.Scientific reports · 2023Article
- Systematic review and meta-analysis on patented and non-patented vitrification processes to ovarian tissue reported between 2000 and 2021.Animal reproduction · 2023Review
- Biomolecular Pathways of Cryoinjuries in Low-Temperature Storage for Mammalian Specimens.Bioengineering (Basel, Switzerland) · 2022Review
- Detailed Morphological Analysis of Cryoinjury in Human Ovarian Tissue Following Vitrification or Slow Freezing.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Fertility preservation strategies for cancerous women: An updated review.Turkish journal of obstetrics and gynecology · 2022Article
- Comparison between slow freezing and vitrification of ovarian tissue cryopreservation in assigned female at birth transgender people receiving testosterone therapy: data on histological and viability parameters.Journal of assisted reproduction and genetics · 2022Article
- Methods of Ovarian Tissue Cryopreservation: Is Vitrification Superior to Slow Freezing?-Ovarian Tissue Freezing Methods.Reproductive sciences (Thousand Oaks, Calif.) · 2021Review
- The Effects of Negative Elements in Environment and Cancer on Female Reproductive System.Advances in experimental medicine and biology · 2021Article
- Folliculogenesis-Associated Genes Expression in Human Vitrified Ovarian Tissue after Xenotransplantation in γ-Irradiated Mice.Cell journal · 2020Article
- The growth and development conditions in mouse offspring derived from ovarian tissue cryopreservation and orthotopic transplantation.Journal of assisted reproduction and genetics · 2020Article
- Vitrification freezing of large ovarian tissue in the human body.Journal of ovarian research · 2019Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCryopreservation of ovarian tissue can be used to preserve the fertility of patients who are about to receive treatment(s) that could compromise their future ovarian function. Here we evaluate the effectiveness of a vitrification protocol by carrying out a systematic comparison with a conventional slow-freezing method on human ovarian tissue.
methodsHuman ovarian samples (mean age 28.0 ± 1.1 years) were processed in parallel for each cryopreservation procedure: vitrification and slow-freezing. Following warming/thawing, histological observations and a TUNEL assay in ovarian follicles were performed and compared to unfrozen control.
resultsBoth cryopreservation protocols gave comparable histological outcomes. Percentage of intact follicles was 83.6 % following vitrification in a 1.5 M 1,2-propanediol (PrOH), 1.5 M ethylene glycol (EG) and 0.5 M raffinose solution, 80.7 % after slow-freezing in 1.5 M PrOH and 0.025 M raffinose, and 99.6 % in fresh tissue. Follicle density was unchanged by vitrification (0.6 follicles/mm2) or slow-freezing (0.5 follicles/mm2) compared to fresh tissue (0.7 follicles/mm2). Percentage of follicles with DNA fragmentation was not statistically different in vitrified (20.8 %) or slow-frozen (31.3 %) tissues compared to the unfrozen control (35.0 %). There was no difference in proportion of stroma cells with DNA fragmentation in vitrified (6.4 %) and slow-frozen (3.7 %) tissues compared to unfrozen tissue (4.2 %).
conclusionsThis vitrification protocol enables good preservation of ovarian quality post-warming. The evaluation of endocrine function after vitrification need to be perform in a higher cohort to evaluate if this protocol may offer a relevant alternative to conventional slow-freezing for the cryopreservation of human ovarian tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.