ArticleScientific reports2015
Phosphorylation of Beet black scorch virus coat protein by PKA is required for assembly and stability of virus particles.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- The multidimensional significance of virus-host protein interactions and their implications in the antiviral defense of plants.Stress biology · 2026Review
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- Zn2+-dependent association of cysteine-rich protein with virion orchestrates morphogenesis of rod-shaped viruses.PLoS pathogens · 2024Article
- Five questions on the cell-to-cell movement ofBBA advances · 2024Article
- Transcriptome analysis of genes involved in the pathogenesis mechanism of potato virus Y in potato cultivar YouJin.Frontiers in microbiology · 2024Article
- Phosphorylated viral protein evades plant immunity through interfering the function of RNA-binding protein.PLoS pathogens · 2022Article
- Phosphorylation of plant virus proteins: Analysis methods and biological functions.Frontiers in microbiology · 2022Review
- Plant viral proteins and fibrillarin: the link to complete the infective cycle.Molecular biology reports · 2021Review
- Article
- Casein Kinase 1 Regulates Cytorhabdovirus Replication and Transcription by Phosphorylating a Phosphoprotein Serine-Rich Motif.The Plant cell · 2020Article
- Article
- Positive selection and recombination shaped the large genetic differentiation of Beet black scorch virus population.PloS one · 2019Article
- Phosphorylation coexists with O-GlcNAcylation in a plant virus protein and influences viral infection.Molecular plant pathology · 2018Article
- Proteotype profiling unmasks a viral signalling network essential for poxvirus assembly and transcriptional competence.Nature microbiology · 2018Article
- Hsc70-2 is required for Beet black scorch virus infection through interaction with replication and capsid proteins.Scientific reports · 2018Article
- Cucumber mosaic virus coat protein modulates the accumulation of 2b protein and antiviral silencing that causes symptom recovery in planta.PLoS pathogens · 2017Article
- The Barley stripe mosaic virus γb protein promotes chloroplast-targeted replication by enhancing unwinding of RNA duplexes.PLoS pathogens · 2017Article
- Phosphorylation of the Brome Mosaic Virus Capsid Regulates the Timing of Viral Infection.Journal of virology · 2016Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant virus coat proteins (CPs) play a fundamental role in protection of genomic RNAs, virion assembly, and viral movement. Although phosphorylation of several CPs during virus infection have been reported, little information is available about CP phosphorylation of the spherical RNA plant viruses. Here, we demonstrate that the CP of Beet black scorch virus (BBSV), a member of the genus Necrovirus, can be phosphorylated at threonine-41 (T41) by cAMP-dependent protein kinase (PKA)-like kinase in vivo and in vitro. Mutant viruses containing a T41A non-phosphorylatable alanine substitution, and a T41E glutamic acid substitution to mimic threonine phosphorylation were able to replicate but were unable to move systemically in Nicotiana benthamiana. Interestingly, the T41A and T41E mutants generated unstable 17 nm virus-like particles that failed to package viral genomic (g) RNA, compared with wild-type BBSV with 30 nm virions during viral infection in N. benthamiana. Further analyses showed that the T41 mutations had little effect on the gRNA-binding activity of the CP. Therefore, we propose a model whereby CP phosphorylation plays an essential role in long-distance movement of BBSV that involves formation of stable virions.
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