ArticleScientific reports2015
Scattering of Sculpted Light in Intact Brain Tissue, with implications for Optogenetics.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Optogenetic interrogation of the zebrafish lateral line reveals brain-wide neural circuits involved in pattern separation.iScience · 2025Article
- KThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Optical trapping with holographically structured light for single-cell studies.Biophysics reviews · 2023Review
- Optical tweezers across scales in cell biology.Trends in cell biology · 2022Review
- Development of optically controlled "living electrodes" with long-projecting axon tracts for a synaptic brain-machine interface.Science advances · 2021Article
- Red Light Optogenetics in Neuroscience.Frontiers in cellular neuroscience · 2021Review
- Functional emergence of a column-like architecture in layer 5 of mouse somatosensory cortex in vivo.The journal of physiological sciences : JPS · 2019Article
- Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties.Scientific reports · 2018Article
- NeuroMorphoVis: a collaborative framework for analysis and visualization of neuronal morphology skeletons reconstructed from microscopy stacks.Bioinformatics (Oxford, England) · 2018Article
- Optogenetically controlled protein kinases for regulation of cellular signaling.Chemical Society reviews · 2018Review
- Optical trapping of otoliths drives vestibular behaviours in larval zebrafish.Nature communications · 2017Article
- Bio-physically plausible visualization of highly scattering fluorescent neocortical models for in silico experimentation.BMC bioinformatics · 2017Article
- Hypothalamic Projections to the Optic Tectum in Larval Zebrafish.Frontiers in neuroanatomy · 2017Article
- Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.Nature protocols · 2016Article
- Stereotyped initiation of retinal waves by bipolar cells via presynaptic NMDA autoreceptors.Nature communications · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optogenetics uses light to control and observe the activity of neurons, often using a focused laser beam. As brain tissue is a scattering medium, beams are distorted and spread with propagation through neural tissue, and the beam's degradation has important implications in optogenetic experiments. To address this, we present an analysis of scattering and loss of intensity of focused laser beams at different depths within the brains of zebrafish larvae. Our experimental set-up uses a 488 nm laser and a spatial light modulator to focus a diffraction-limited spot of light within the brain. We use a combination of experimental measurements of back-scattered light in live larvae and computational modelling of the scattering to determine the spatial distribution of light. Modelling is performed using the Monte Carlo method, supported by generalised Lorenz-Mie theory in the single-scattering approximation. Scattering in areas rich in cell bodies is compared to that of regions of neuropil to identify the distinct and dramatic contributions that cell nuclei make to scattering. We demonstrate the feasibility of illuminating individual neurons, even in nucleus-rich areas, at depths beyond 100 μm using a spatial light modulator in combination with a standard laser and microscope optics.
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