ArticleNature communications2015
Two-dimensional isobutyl acetate production pathways to improve carbon yield.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 68 citations in OpenAlex.
- Metabolic rewiring for enhanced itaconate yield through heterotrophic COJournal of biological engineering · 2026Article
- Enhanced anti-EpCAM ScFv production and reduced acetate overflow in a BW25113-derived E. coli strain with ArcA and Pka deletions.Microbial cell factories · 2025Article
- CRISPRi-mediated metabolic switch enables concurrent aerobic and synthetic anaerobic fermentations in engineered consortium.Nature communications · 2024Article
- Perspectives for Using COBioengineering (Basel, Switzerland) · 2023Review
- Growth-coupled anaerobic production of isobutanol from glucose in minimal medium with Escherichia coli.Biotechnology for biofuels and bioproducts · 2023Article
- A novel strategy for L-arginine production in engineered Escherichia coli.Microbial cell factories · 2023Article
- Alcohol acyltransferases for the biosynthesis of esters.Biotechnology for biofuels and bioproducts · 2023Review
- Metabolic engineering using acetate as a promising building block for the production of bio-based chemicals.Engineering microbiology · 2022Review
- Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate.Microbial cell factories · 2022Article
- Molecular characterization of the Saccharomycopsis fibuligera ATF genes, encoding alcohol acetyltransferase for volatile acetate ester formation.Journal of microbiology (Seoul, Korea) · 2021Article
- Saccharomyces cerevisiae Atf1p is an alcohol acetyltransferase and a thioesterase in vitro.Yeast (Chichester, England) · 2017Article
- Engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using unrelated carbon sources.Scientific reports · 2016Article
- Metabolic Engineering Strategies for Co-Utilization of Carbon Sources in Microbes.Bioengineering (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For an economically competitive biological process, achieving high carbon yield of a target chemical is crucial. In biochemical production, pyruvate and acetyl-CoA are primary building blocks. When sugar is used as the sole biosynthetic substrate, acetyl-CoA is commonly generated by pyruvate decarboxylation. However, pyruvate decarboxylation during acetyl-CoA formation limits the theoretical maximum carbon yield (TMCY) by releasing carbon, and in some cases also leads to redox imbalance. To avoid these problems, we describe here the construction of a metabolic pathway that simultaneously utilizes glucose and acetate. Acetate is utilized to produce acetyl-CoA without carbon loss or redox imbalance. We demonstrate the utility of this approach for isobutyl acetate (IBA) production, wherein IBA production with glucose and acetate achieves a higher carbon yield than with either sole carbon source. These results highlight the potential for this multiple carbon source approach to improve the TMCY and balance redox in biosynthetic pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.