ArticleClinical and vaccine immunology : CVI2015
IP-10 Is a Sensitive Biomarker of Antigen Recognition in Whole-Blood Stimulation Assays Used for the Diagnosis of Mycobacterium bovis Infection in African Buffaloes (Syncerus caffer).
Article in Clinical and vaccine immunology : CVI, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Diagnosis of tuberculosis in wildlife: a systematic review.Veterinary research · 2021Pooled it
- Exploring specific biomarkers in blood forFrontiers in microbiology · 2026Article
- Studies on the immunological biomarkers of bovine tuberculosis in naturally infected cattle.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Development and characterization of monoclonal antibodies specific for bovine IP-10.Veterinary research · 2025Article
- Exploring potential cytokine profiles as diagnostic biomarkers for brucellosis in Mediterranean Buffaloes.Frontiers in veterinary science · 2025Article
- Cytokines as potential biomarkers for the diagnosis ofFrontiers in veterinary science · 2024Article
- High-Specificity Test Algorithm for Bovine Tuberculosis Diagnosis in African Buffalo (Pathogens (Basel, Switzerland) · 2022Article
- Detection of Mycobacterium tuberculosis complex DNA in oronasal swabs from infected African buffaloes (Syncerus caffer).Scientific reports · 2022Article
- Prediction of Johne's disease state based on quantification of T cell markers and their interaction with macrophages in the bovine intestine.Veterinary research · 2021Article
- Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes.Scientific reports · 2021Article
- Cell-Mediated Immunological Biomarkers and Their Diagnostic Application in Livestock and Wildlife Infected WithFrontiers in immunology · 2021Review
- The VetMAX™ M. tuberculosis complex PCR kit detects MTBC DNA in antemortem and postmortem samples from white rhinoceros (Ceratotherium simum), African elephants (Loxodonta africana) and African buffaloes (Syncerus caffer).BMC veterinary research · 2020Article
- Biomarkers for Detecting Resilience against Mycobacterial Disease in Animals.Infection and immunity · 2019Review
- Interleukin 8 and Pentaxin (C-Reactive Protein) as Potential New Biomarkers of Bovine Tuberculosis.Journal of clinical microbiology · 2019Article
- Immune response biomarkers in human and veterinary research.Comparative immunology, microbiology and infectious diseases · 2018Review
- Limitations of Using IL-17A and IFN-γ-Induced Protein 10 to Detect Bovine Tuberculosis.Frontiers in veterinary science · 2018Article
- TB Control in Humans and Animals in South Africa: A Perspective on Problems and Successes.Frontiers in veterinary science · 2018Article
- Development and evaluation of a diagnostic cytokine-release assay for Mycobacterium suricattae infection in meerkats (Suricata suricatta).BMC veterinary research · 2017Article
- Positive Correlation between IP-10 and IFN-BioMed research international · 2017Article
- Interleukin-17A as a Biomarker for Bovine Tuberculosis.Clinical and vaccine immunology : CVI · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
African buffaloes (Syncerus caffer) are maintenance hosts of Mycobacterium bovis, the causative agent of bovine tuberculosis. They act as reservoirs of this infection for a wide range of wildlife and domestic species, and the detection of infected animals is important to control the geographic spread and transmission of the disease. Interferon gamma (IFN-γ) release assays (IGRAs) utilizing pathogen-derived peptide antigens are highly specific tests of M. bovis infection; however, the diagnostic sensitivities of these assays are suboptimal. We evaluated the diagnostic utility of measuring antigen-dependent interferon gamma-induced protein 10 (IP-10) release as an alternative to measuring IFN-γ levels. M. bovis-exposed buffaloes were tested using the Bovigam PC-EC and Bovigam PC-HP assays and a modified QuantiFERON TB-Gold (mQFT) assay. IP-10 was measured in the harvested plasma and was produced in significantly greater abundance in response to M. bovis antigens in Bovigam-positive than in Bovigam-negative animals. For each assay, using the Bovigam results as a reference, receiver operating characteristic curve analysis was done to determine diagnostically relevant cutoff values for IP-10. Thereafter, mQFT test results derived from measurement of IP-10 and IFN-γ were compared and a larger number of Bovigam-positive animals were detected using IP-10 as a diagnostic marker. Moreover, using IP-10, agreement between the mQFT assay and the Bovigam assays was increased, while the excellent agreement between the Bovigam assays was retained. We conclude that IP-10 is a sensitive marker of antigen recognition and that measurement of this cytokine in antigen-stimulated whole blood might increase the sensitivity of conventional IGRAs in African buffaloes.
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