Evidence map›Paper›PMID 26107446›Full record

ArticleRevista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular

Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody.

Chun-Li Song, Qian Li, Yun-Peng Yu, Guan Wang, Jin-Peng Wang, Yang Lu, Ji-Chang Zhang, Hong-Ying Diao, Jian-Gen Liu, Yi-Hang Liu and 4 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. [Endothelial injury and its repair strategies after intravascular stents implantation].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 1 institution in 1 country.

Chun-Li SongThe Second Hospital, Jilin University, Changchun, Jilin, China.
Qian LiThe Second Hospital, Jilin University, Changchun, Jilin, China.
Yun-Peng YuThe Second Hospital, Jilin University, Changchun, Jilin, China.
Guan WangThe Second Hospital, Jilin University, Changchun, Jilin, China.
Jin-Peng WangThe Second Hospital, Jilin University, Changchun, Jilin, China.
Yang LuThe Second Hospital, Jilin University, Changchun, Jilin, China.
Ji-Chang ZhangThe Second Hospital, Jilin University, Changchun, Jilin, China.
Hong-Ying DiaoThe Second Hospital, Jilin University, Changchun, Jilin, China.
Jian-Gen LiuThe Second Hospital, Jilin University, Changchun, Jilin, China.
Yi-Hang LiuThe Second Hospital, Jilin University, Changchun, Jilin, China.
Jia LiuThe Second Hospital, Jilin University, Changchun, Jilin, China.
Ying LiThe Second Hospital, Jilin University, Changchun, Jilin, China.
Dan CaiThe Second Hospital, Jilin University, Changchun, Jilin, China.
Bin LiuThe Second Hospital, Jilin University, Changchun, Jilin, China.
Second Affiliated Hospital of Jilin University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionIntravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultaneously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel.

methodsRound 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensation reaction of dopamine and heparin using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V)10 group. Eight sheets from the D-(H-V)10 group were coated with anti-CD34 antibody and termed as the D-(H-V)10-A group. Immunofluorescence assay and ELISA were used to evaluate whether the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody.

resultsThe results of immunofluorescence assay and ELISA showed that VEGF could be detected in the D-(H-V)10 and D-(H-V)10-A group, suggesting the steel sheets were successfully covered with VEGF. Anti-CD34 antibody could only be observed in the D-(H-V)10-A group, which was the only group coated with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody.

conclusionOur study developed a method to simultaneously coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy.

Indexed as

Drug-Eluting StentsAntigens, CD34Coated Materials, BiocompatibleCoronary RestenosisEnzyme-Linked Immunosorbent AssayEthyldimethylaminopropyl CarbodiimideFluorescent Antibody TechniqueHumansMaterials TestingReproducibility of ResultsSerum Albumin, BovineStainless SteelTime FactorsVascular Endothelial Growth Factor AAntigens, CD34Coated Materials, BiocompatibleEthyldimethylaminopropyl CarbodiimideSerum Albumin, BovineStainless SteelVascular Endothelial Growth Factor A

Identifiers

PMID26107446
PMCPMC4462960
OpenAlexW1808031906

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.