Evidence map›Paper›PMID 26107351›Full record

ArticlePloS one2015

Annotation of the Protein Coding Regions of the Equine Genome.

Matthew S Hestand, Theodore S Kalbfleisch, Stephen J Coleman, Zheng Zeng, Jinze Liu, Ludovic Orlando, James N MacLeod

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 35 citations in OpenAlex.

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  14. Metabolic engineering ofBiotechnology for biofuels · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Matthew S HestandMaxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, United States of America.
Theodore S KalbfleischBiochemistry and Molecular Biology Department, School of Medicine, University of Louisville, Louisville, Kentucky, United States of America.
Stephen J ColemanMaxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, United States of America.
Zheng ZengDepartment of Computer Science, University of Kentucky, Lexington, Kentucky, United States of America.
Jinze LiuDepartment of Computer Science, University of Kentucky, Lexington, Kentucky, United States of America.
Ludovic OrlandoCentre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark.
James N MacLeodMaxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, United States of America.
University of Kentucky · USUniversity of Copenhagen · DKUniversity of Louisville · US

Funding

WKU Lead Faculty AwardP20GM103436 · NIGMS · UNIVERSITY OF LOUISVILLE · PI ERIC C ROUCHKA · 2012 to 2026
$60.1M
ZEBRAFISH VISUAL FUNCTION RECOVERY FOLLOWING OPTIC NERVE DAMAGEP20RR016481 · NCRR · UNIVERSITY OF LOUISVILLE · PI BARTON, HAZEL A · 2001 to 2011
$34.8M
Unlocking transcript diversity via differential analyses of splice graphsR01HG006272 · NHGRI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI LIU, JINZE, PRINS, JAN F · 2012 to 2014
$1.2M
NCRR NIH HHS 5P20RR016481-09NCRR NIH HHS P20 RR016481NHGRI NIH HHS R01 HG006272NIGMS NIH HHS P20 GM103436
6 · The paper itself

Abstract

Current gene annotation of the horse genome is largely derived from in silico predictions and cross-species alignments. Only a small number of genes are annotated based on equine EST and mRNA sequences. To expand the number of equine genes annotated from equine experimental evidence, we sequenced mRNA from a pool of forty-three different tissues. From these, we derived the structures of 68,594 transcripts. In addition, we identified 301,829 positions with SNPs or small indels within these transcripts relative to EquCab2. Interestingly, 780 variants extend the open reading frame of the transcript and appear to be small errors in the equine reference genome, since they are also identified as homozygous variants by genomic DNA resequencing of the reference horse. Taken together, we provide a resource of equine mRNA structures and protein coding variants that will enhance equine and cross-species transcriptional and genomic comparisons.

Indexed as

Molecular Sequence AnnotationAnimalsBase SequenceGenomeHorsesOpen Reading FramesRNA, MessengerRNA, Messenger

Identifiers

PMID26107351
PMCPMC4481266
OpenAlexW1602804419

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.