ArticleRNA (New York, N.Y.)2015
Insights into transcription termination of Hfq-binding sRNAs of Escherichia coli and characterization of readthrough products.
Article in RNA (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- An internal loop region is responsible for inherent target specificity of bacterial cold-shock proteins.RNA (New York, N.Y.) · 2024Article
- What goes up must come down: off switches for regulatory RNAs.Genes & development · 2024Review
- The read-through transcription-mediated autoactivation circuit for virulence regulator expression drives robust type III secretion system 2 expression in Vibrio parahaemolyticus.PLoS pathogens · 2024Article
- Article
- Mapping the Complex Transcriptional Landscape of the Phytopathogenic Bacterium Dickeya dadantii.mBio · 2022Article
- Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA.Nature communications · 2021Article
- Synthetic small regulatory RNAs in microbial metabolic engineering.Applied microbiology and biotechnology · 2021Review
- Genome-wide identification of Azospirillum brasilense Sp245 small RNAs responsive to nitrogen starvation and likely involvement in plant-microbe interactions.BMC genomics · 2020Article
- Prevalence of small base-pairing RNAs derived from diverse genomic loci.Biochimica et biophysica acta. Gene regulatory mechanisms · 2020Review
- Trans-Acting Small RNAs and Their Effects on Gene Expression inEcoSal Plus · 2020Review
- Small RNA Mcr11 requires the transcription factor AbmR for stable expression and regulates genes involved in the central metabolism of Mycobacterium tuberculosis.Molecular microbiology · 2020Article
- Stochastic Analysis Demonstrates the Dual Role of Hfq in ChaperoningFrontiers in molecular biosciences · 2020Article
- AmBio · 2019Article
- Mechanism and physiological significance of autoregulation of theRNA (New York, N.Y.) · 2019Article
- Regulation of Transcription Termination of Small RNAs and by Small RNAs: Molecular Mechanisms and Biological Functions.Frontiers in cellular and infection microbiology · 2019Review
- SMRT-Cappable-seq reveals complex operon variants in bacteria.Nature communications · 2018Article
- Sponges and Predators in the Small RNA World.Microbiology spectrum · 2018Article
- Role of the terminator hairpin in the biogenesis of functional Hfq-binding sRNAs.RNA (New York, N.Y.) · 2017Article
- Hfq and three Hfq-dependent small regulatory RNAs-MgrR, RyhB and McaS-coregulate the locus of enterocyte effacement in enteropathogenic Escherichia coli.Pathogens and disease · 2017Article
- A Genome-Wide Prediction and Identification of Intergenic Small RNAs by Comparative Analysis inFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genes encoding Hfq-dependent sRNAs possess a typical Rho-independent transcription terminator. Here, we have studied the molecular events occurring at Rho-independent terminators of sRNA genes, focusing on two well-characterized Hfq-binding sRNAs, SgrS and RyhB. We constructed several hybrid genes in which the DNA sequence corresponding to a strong Rho-independent terminator was placed just downstream from the Rho-independent terminators of sRNA genes. By using this system, we demonstrate that transcripts frequently read through the Rho-independent terminators of sgrS and ryhB in normally growing cells. We show that Hfq does not affect the transcriptional readthrough event itself. We also find that the readthrough products no longer bind to Hfq in vivo. We have developed a competition assay based on a biotin-streptavidin system to analyze the interaction of Hfq and a particular RNA molecule in vitro. By using this method, we verify that the 3'-extended form of SgrS does not bind to Hfq in vitro. Finally, we demonstrate that transcription termination is significantly enhanced under stress conditions where transcription initiation of sRNA genes on the chromosome is induced. We conclude that the production of sRNAs is regulated not only at the step of transcription initiation but also at the step of transcription termination. The mechanism by which transcription termination is enhanced under stress conditions remains to be understood.
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