ArticleProceedings of the National Academy of Sciences of the United States of America2015
Trp triad-dependent rapid photoreduction is not required for the function of Arabidopsis CRY1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 64 citations in OpenAlex.
- 'Seeing' the electromagnetic spectrum: spotlight on the cryptochrome photocycle.Frontiers in plant science · 2024Review
- NUA positively regulates plant immunity by coordination with ESD4 to deSUMOylate TPR1 in Arabidopsis.The New phytologist · 2024Article
- Blue light receptor CRY1 regulates HSFA1d nuclear localization to promote plant thermotolerance.Cell reports · 2023Article
- Arabidopsis cryptochrome 2 forms photobodies with TCP22 under blue light and regulates the circadian clock.Nature communications · 2022Article
- Article
- The blue light receptor CRY1 interacts with GID1 and DELLA proteins to repress gibberellin signaling and plant growth.Plant communications · 2021Article
- Towards the Idea of Molecular Brains.International journal of molecular sciences · 2021Review
- Bioluminescence and Photoreception in Unicellular Organisms: Light-Signalling in a Bio-Communication Perspective.International journal of molecular sciences · 2021Review
- Cryptochromes: Photochemical and structural insight into magnetoreception.Protein science : a publication of the Protein Society · 2021Review
- Identification of a bacterial-type ATP-binding cassette transporter implicated in aluminum tolerance in sweet sorghum (Plant signaling & behavior · 2021Article
- Structural insights into photoactivation of plant Cryptochrome-2.Communications biology · 2021Article
- Plant Cryptochromes Illuminated: A Spectroscopic Perspective on the Mechanism.Frontiers in chemistry · 2021Review
- Regulatory Impact of the C-Terminal Tail on Charge Transfer Pathways inMolecules (Basel, Switzerland) · 2020Article
- ThePhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- The oligomeric structures of plant cryptochromes.Nature structural & molecular biology · 2020Article
- Structural insights into BIC-mediated inactivation of Arabidopsis cryptochrome 2.Nature structural & molecular biology · 2020Article
- A structural view of plant CRY2 photoactivation and inactivation.Nature structural & molecular biology · 2020Article
- Mechanisms of Cryptochrome-Mediated Photoresponses in Plants.Annual review of plant biology · 2020Article
- Cryptochrome-mediated blue-light signalling modulates UVR8 photoreceptor activity and contributes to UV-B tolerance in Arabidopsis.Nature communications · 2020Article
- The Universally Conserved Residues Are Not Universally Required for Stable Protein Expression or Functions of Cryptochromes.Molecular biology and evolution · 2020Article
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9 authors at 4 institutions in 2 countries.
Funding
Abstract
Cryptochromes in different evolutionary lineages act as either photoreceptors or light-independent transcription repressors. The flavin cofactor of both types of cryptochromes can be photoreduced in vitro by electron transportation via three evolutionarily conserved tryptophan residues known as the "Trp triad." It was hypothesized that Trp triad-dependent photoreduction leads directly to photoexcitation of cryptochrome photoreceptors. We tested this hypothesis by analyzing mutations of Arabidopsis cryptochrome 1 (CRY1) altered in each of the three Trp-triad tryptophan residues (W324, W377, and W400). Surprisingly, in contrast to a previous report all photoreduction-deficient Trp-triad mutations of CRY1 remained physiologically and biochemically active in Arabidopsis plants. ATP did not enhance rapid photoreduction of the wild-type CRY1, nor did it rescue the defective photoreduction of the CRY1(W324A) and CRY1(W400F) mutants that are photophysiologically active in vivo. The lack of correlation between rapid flavin photoreduction or the effect of ATP on the rapid flavin photoreduction and the in vivo photophysiological activities of plant cryptochromes argues that the Trp triad-dependent photoreduction is not required for the function of cryptochromes and that further efforts are needed to elucidate the photoexcitation mechanism of cryptochrome photoreceptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.