Evidence map›Paper›PMID 26104913›Full record

ArticleMucosal immunology2016

Increased levels of inflammatory cytokines in the female reproductive tract are associated with altered expression of proteases, mucosal barrier proteins, and an influx of HIV-susceptible target cells.

Kelly B Arnold, Adam Burgener, Kenzie Birse, Laura Romas, Laura J Dunphy, Kamnoosh Shahabi, Max Abou, Garrett R Westmacott, Stuart McCorrister, Jessie Kwatampora and 9 more

Registry-linked trialOpen access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Mucosal immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02878564 (Effect of Schistosomiasis Mansoni and Its Treatment on HIV Susceptibility and Female Genital Immunology), which is not on this map. Cited by 183 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
183citing papers in PubMed, 2 pooled it
8.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02878564 phase4completednot on this map

Effect of Schistosomiasis Mansoni and Its Treatment on HIV Susceptibility and Female Genital Immunology

TypeinterventionalSponsorUniversity of TorontoRan2016 to 2016Enrolled34ConditionsSchistosomiasis Mansoni, HIVArmsPraziquantel
3 · Its place in the literature

Who cites it

183 citing papers in PubMed, 2 syntheses or guidelines pooled it, 246 citations in OpenAlex.

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  5. Genital inflammatory status and the innate immune response to contraceptive initiation.American journal of reproductive immunology (New York, N.Y. : 1989) · 2022
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  7. Persistent, Asymptomatic Colonization withMicrobiology spectrum · 2022
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123 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 8 institutions in 5 countries.

Kelly B ArnoldDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Adam BurgenerNational HIV and Retrovirology Laboratory, JC Wilt Infectious Disease Research Centre, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
Kenzie BirseNational HIV and Retrovirology Laboratory, JC Wilt Infectious Disease Research Centre, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
Laura RomasNational HIV and Retrovirology Laboratory, JC Wilt Infectious Disease Research Centre, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
Laura J DunphyDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Kamnoosh ShahabiDepartment of Medicine, University of Toronto, Toronto, Canada.
Max AbouNational HIV and Retrovirology Laboratory, JC Wilt Infectious Disease Research Centre, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
Garrett R WestmacottNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
Stuart McCorristerNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
Jessie KwatamporaDepartment of Medical Microbiology, University of Nairobi, Nairobi, Kenya.
Billy NyangaDepartment of Medical Microbiology, University of Nairobi, Nairobi, Kenya.
Joshua KimaniDepartment of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Lindi MassonInstitute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town, Cape Town, South Africa.
Lenine J LiebenbergCentre for the AIDS Programme of Research in South Africa (CAPRISA), Durban, South Africa.
Salim S Abdool KarimCentre for the AIDS Programme of Research in South Africa (CAPRISA), Durban, South Africa.
Jo-Ann S PassmoreInstitute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town, Cape Town, South Africa.
Douglas A LauffenburgerDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Rupert KaulDepartment of Medicine, University of Toronto, Toronto, Canada.
Lyle R McKinnonDepartment of Medicine, University of Toronto, Toronto, Canada.
Public Health Agency of Canada · CAUniversity of Nairobi · KECentre for the AIDS Programme of Research in South Africa · ZAMassachusetts Institute of Technology · USUniversity of Manitoba · CAColumbia University · USNational Health Laboratory Service · ZAUniversity of Toronto · CA

Funding

Canadian Institutes of Health Research MOP-89983
6 · The paper itself

Abstract

Elevated inflammatory cytokines (EMCs) at mucosal surfaces have been associated with HIV susceptibility, but the underlying mechanisms remain unclear. We characterized the soluble mucosal proteome associated with elevated cytokine expression in the female reproductive tract. A scoring system was devised based on the elevation (upper quartile) of at least three of seven inflammatory cytokines in cervicovaginal lavage. Using this score, HIV-uninfected Kenyan women were classified as either having EMC (n=28) or not (n=68). Of 455 proteins quantified in proteomic analyses, 53 were associated with EMC (5% false discovery rate threshold). EMCs were associated with proteases, cell motility, and actin cytoskeletal pathways, whereas protease inhibitor, epidermal cell differentiation, and cornified envelope pathways were decreased. Multivariate analysis identified an optimal signature of 16 proteins that distinguished the EMC group with 88% accuracy. Three proteins in this signature were neutrophil-associated proteases that correlated with many cytokines, especially GM-CSF (granulocyte-macrophage colony-stimulating factor), IL-1β (interleukin-1β), MIP-3α (macrophage inflammatory protein-3α), IL-17, and IL-8. Gene set enrichment analyses implicated activated immune cells; we verified experimentally that EMC women had an increased frequency of endocervical CD4(+) T cells. These data reveal strong linkages between mucosal cytokines, barrier function, proteases, and immune cell movement, and propose these as potential mechanisms that increase risk of HIV acquisition.

Indexed as

AdultCD4-Positive T-LymphocytesCell MovementChemokine CCL20CytokinesCytoskeletal ProteinsDisease SusceptibilityFemaleGene Expression ProfilingGene Expression RegulationGenitalia, FemaleGranulocyte-Macrophage Colony-Stimulating FactorHIV InfectionsHumansInterleukin-17Interleukin-1betaCCL20 protein, humanChemokine CCL20CytokinesCytoskeletal ProteinsGranulocyte-Macrophage Colony-Stimulating FactorInterleukin-17Interleukin-1betaInterleukin-8Peptide Hydrolases

Identifiers

PMID26104913
OpenAlexW2435715880

What OpenQuestion holds

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Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.