ArticleNature communications2015
Digital microfluidic immunocytochemistry in single cells.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- Orbital Electrowetting: From Continuous Droplet Transport to Programmable Microfluidics.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Nucleic acid amplification tests in digital microfluidics: the promise of next-generation point-of-care diagnostics.Microsystems & nanoengineering · 2025Review
- Droplet acoustofluidics: Recent progress and challenges.Biomicrofluidics · 2025Review
- Article
- On-chip dielectrophoretic single-cell manipulation.Microsystems & nanoengineering · 2024Review
- Open and closed microfluidics for biosensing.Materials today. Bio · 2024Review
- Drug screening on digital microfluidics for cancer precision medicine.Nature communications · 2024Article
- Beyond single cells: microfluidics empowering multiomics analysis.Analytical and bioanalytical chemistry · 2024Review
- Massive and efficient encapsulation of single cells in monodisperse droplets and collagen-alginate microgels using a microfluidic device.Frontiers in bioengineering and biotechnology · 2023Article
- Article
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Recent advances in critical nodes of embryo engineering technology.Theranostics · 2021Review
- Digital-WGS: Automated, highly efficient whole-genome sequencing of single cells by digital microfluidics.Science advances · 2020Article
- Digital microfluidic isolation of single cells for -Omics.Nature communications · 2020Article
- Article
- Profiling Cell Signaling Networks at Single-cell Resolution.Molecular & cellular proteomics : MCP · 2020Review
- Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers.Micromachines · 2020Article
- Separation-encoded microparticles for single-cell western blotting.Lab on a chip · 2020Article
- In Situ Single-Cell Western Blot on Adherent Cell Culture.Angewandte Chemie (International ed. in English) · 2019Review
- Rapid generation of chemical combinations on a magnetic digital microfluidic array.RSC advances · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a new technique called Digital microfluidic Immunocytochemistry in Single Cells (DISC). DISC automates protocols for cell culture, stimulation and immunocytochemistry, enabling the interrogation of protein phosphorylation on pulsing with stimulus for as little as 3 s. DISC was used to probe the phosphorylation states of platelet-derived growth factor receptor (PDGFR) and the downstream signalling protein, Akt, to evaluate concentration- and time-dependent effects of stimulation. The high time resolution of the technique allowed for surprising new observations-for example, a 10 s pulse stimulus of a low concentration of PDGF is sufficient to cause >30% of adherent fibroblasts to commit to Akt activation. With the ability to quantitatively probe signalling events with high time resolution at the single-cell level, we propose that DISC may be an important new technique for a wide range of applications, especially for screening signalling responses of a heterogeneous cell population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.