ArticleeLife2015
A genetic toolkit for tagging intronic MiMIC containing genes.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed.
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- Morphological and functional convergence of visual projection neurons from diverse neurogenic origins in Drosophila.Nature communications · 2025Article
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- A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans.PLoS genetics · 2025Article
- A simple MiMIC-based approach for tagging endogenous genes to visualise live transcription in Drosophila.Development (Cambridge, England) · 2024Article
- Article
- Transient caspase-mediated activation of caspase-activated DNase causes DNA damage required for phagocytic macrophage differentiation.Cell reports · 2024Article
- De novo variants in FRYL are associated with developmental delay, intellectual disability, and dysmorphic features.American journal of human genetics · 2024Article
- Cellular and molecular organization of the Drosophila foregut.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Loss of the endoplasmic reticulum protein Tmem208 affects cell polarity, development, and viability.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
34 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
Abstract
Previously, we described a large collection of Minos-Mediated Integration Cassettes (MiMICs) that contain two phiC31 recombinase target sites and allow the generation of a new exon that encodes a protein tag when the MiMIC is inserted in a codon intron (Nagarkar-Jaiswal et al., 2015). These modified genes permit numerous applications including assessment of protein expression pattern, identification of protein interaction partners by immunoprecipitation followed by mass spec, and reversible removal of the tagged protein in any tissue. At present, these conversions remain time and labor-intensive as they require embryos to be injected with plasmid DNA containing the exon tag. In this study, we describe a simple and reliable genetic strategy to tag genes/proteins that contain MiMIC insertions using an integrated exon encoding GFP flanked by FRT sequences. We document the efficiency and tag 60 mostly uncharacterized genes.
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