ArticleBiotechnology for biofuels2015
Optimized inorganic carbon regime for enhanced growth and lipid accumulation in Chlorella vulgaris.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 95 citations in OpenAlex.
- Adaptive and Biochemical Responses of Dictyosphaerium sp. AM-2024a to Environmental Conditions and Microplastic Interactions: Synergy of Biofuel Production With Pollution Mitigation.Biotechnology and applied biochemistry · 2026Article
- Oleaginous MicroalgaFrontiers in bioengineering and biotechnology · 2022Article
- Microalgae as Contributors to Produce Biopolymers.Marine drugs · 2021Review
- Insights into the physiology of Chlorella vulgaris cultivated in sweet sorghum bagasse hydrolysate for sustainable algal biomass and lipid production.Scientific reports · 2021Article
- Boosting Biomass Quantity and Quality by Improved Mixotrophic Culture of the DiatomFrontiers in plant science · 2021Article
- Use of an immobilised thermostableJournal of enzyme inhibition and medicinal chemistry · 2020Article
- Comparison of the Photoautotrophic Growth Regimens ofBiology · 2020Article
- Overexpression of bicarbonate transporters in the marine cyanobacteriumBiotechnology for biofuels · 2020Article
- Species disparity response to mutagenesis of marine yeasts for the potential production of biodiesel.Biotechnology for biofuels · 2019Article
- Application of high-salinity stress for enhancing the lipid productivity of Chlorella sorokiniana HS1 in a two-phase process.Journal of microbiology (Seoul, Korea) · 2018Article
- Mixotrophic transition induced lipid productivity inHeliyon · 2018Article
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6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundLarge-scale algal biofuel production has been limited, among other factors, by the availability of inorganic carbon in the culture medium at concentrations higher than achievable with atmospheric CO2. Life cycle analyses have concluded that costs associated with supplying CO2 to algal cultures are significant contributors to the overall energy consumption.
resultsA two-phase optimal growth and lipid accumulation scenario is presented, which (1) enhances the growth rate and (2) the triacylglyceride (TAG) accumulation rate in the oleaginous Chlorophyte Chlorella vulgaris strain UTEX 395, by growing the organism in the presence of low concentrations of NaHCO3 (5 mM) and controlling the pH of the system with a periodic gas sparge of 5 % CO2 (v/v). Once cultures reached the desired cell densities, which can be "fine-tuned" based on initial nutrient concentrations, cultures were switched to a lipid accumulation metabolism through the addition of 50 mM NaHCO3. This two-phase approach increased the specific growth rate of C. vulgaris by 69 % compared to cultures sparged continuously with 5 % CO2 (v/v); further, biomass productivity (g L(-1) day(-1)) was increased by 27 %. Total biodiesel potential [assessed as total fatty acid methyl ester (FAME) produced] was increased from 53.3 to 61 % (FAME biomass(-1)) under the optimized conditions; biodiesel productivity (g FAME L(-1) day(-1)) was increased by 7.7 %. A bicarbonate salt screen revealed that American Chemical Society (ACS) and industrial grade NaHCO3 induced the highest TAG accumulation (% w/w), whereas Na2CO3 did not induce significant TAG accumulation. NH4HCO3 had a negative effect on cell health presumably due to ammonia toxicity. The raw, unrefined form of trona, NaHCO3∙Na2CO3 (sodium sesquicarbonate) induced TAG accumulation, albeit to a slightly lower extent than the more refined forms of sodium bicarbonate.
conclusionsThe strategic addition of sodium bicarbonate was found to enhance growth and lipid accumulation rates in cultures of C. vulgaris, when compared to traditional culturing strategies, which rely on continuously sparging algal cultures with elevated concentrations of CO2(g). This work presents a two-phased, improved photoautotrophic growth and lipid accumulation approach, which may result in an overall increase in algal biofuel productivity.
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