Evidence map›Paper›PMID 26101352›Full record

ArticleJournal of cell science2015

Analysis of ER-mitochondria contacts using correlative fluorescence microscopy and soft X-ray tomography of mammalian cells.

Kirstin D Elgass, Elizabeth A Smith, Mark A LeGros, Carolyn A Larabell, Michael T Ryan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.

0numbers the graph read from it
0cells of the map it votes in
67citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

67 citing papers in PubMed, 93 citations in OpenAlex.

  1. Review
  2. Review
  3. Subcellular Stress Markers in Epithelial Ovarian Cancer.International journal of molecular sciences · 2025
    Review
  4. Review
  5. DRP1, fission and apoptosis.Cell death discovery · 2025
    Review
  6. Molecular mechanisms of mitochondrial dynamics.Nature reviews. Molecular cell biology · 2025
    Review
  7. Article
  8. Review
  9. Review
  10. Article
  11. Review
  12. Review
  13. Review
  14. Review
  15. Article
  16. Article
  17. Mitochondria-ER Tethering in Neurodegenerative Diseases.Cellular and molecular neurobiology · 2022
    Review
  18. Article
  19. Review
  20. Article

7 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Kirstin D ElgassHudson Institute for Medical Research, Monash Micro Imaging, Monash University, Melbourne 3168, Australia.
Elizabeth A SmithDepartment of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94158, USA National Centre for X-ray Tomography, Advanced Light Source, Berkeley, CA 94720, USA.
Mark A LeGrosDepartment of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94158, USA National Centre for X-ray Tomography, Advanced Light Source, Berkeley, CA 94720, USA.
Carolyn A LarabellDepartment of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94158, USA National Centre for X-ray Tomography, Advanced Light Source, Berkeley, CA 94720, USA Carolyn.Larabell@ucsf.edu Michael.Ryan@monash.edu.
Michael T RyanDepartment of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne 3800, Australia Carolyn.Larabell@ucsf.edu Michael.Ryan@monash.edu.
Monash University · AUUniversity of California, San Francisco · USAdvanced Light Source

Funding

TrainingP41GM103445 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LARABELL, CAROLYN A · 2012 to 2019
$8.2M
NIGMS NIH HHS P41 GM103445NIGMS NIH HHS P41GM103445
6 · The paper itself

Abstract

Mitochondrial fission is important for organelle transport, quality control and apoptosis. Changes to the fission process can result in a wide variety of neurological diseases. In mammals, mitochondrial fission is executed by the GTPase dynamin-related protein 1 (Drp1; encoded by DNM1L), which oligomerizes around mitochondria and constricts the organelle. The mitochondrial outer membrane proteins Mff, MiD49 (encoded by MIEF2) and MiD51 (encoded by MIEF1) are involved in mitochondrial fission by recruiting Drp1 from the cytosol to the organelle surface. In addition, endoplasmic reticulum (ER) tubules have been shown to wrap around and constrict mitochondria before a fission event. Up to now, the presence of MiD49 and MiD51 at ER-mitochondrial division foci has not been established. Here, we combine confocal live-cell imaging with correlative cryogenic fluorescence microscopy and soft x-ray tomography to link MiD49 and MiD51 to the involvement of the ER in mitochondrial fission. We gain further insight into this complex process and characterize the 3D structure of ER-mitochondria contact sites.

Indexed as

AnimalsApoptosisCell Line, TumorChlorocebus aethiopsCOS CellsDynaminsEndoplasmic ReticulumImaging, Three-DimensionalMembrane ProteinsMiceMicroscopy, ConfocalMicroscopy, FluorescenceMitochondriaMitochondrial DynamicsMitochondrial MembranesMitochondrial ProteinsDnm1l protein, mouseDynaminsMembrane ProteinsMiD51 protein, mouseMIEF2 protein, mouseMitochondrial ProteinsReceptors, Cytoplasmic and NuclearConfocal microscopyCorrelative imagingEndoplasmic reticulumMicroscopy imagingMitochondriaMitochondrial fissionSoft X-ray tomography

Identifiers

PMID26101352
PMCPMC4540952
OpenAlexW1897303836

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.