ArticleNucleic acids research2015
ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Omega Nucleic Acids (ΩNA), Ultimate Nucleic Acids for Future Technology.Molecules (Basel, Switzerland) · 2026Review
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- Real-time imaging of RNA polymerase I activity in living human cells.The Journal of cell biology · 2023Article
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- Subcellular Transcriptomics and Proteomics: A Comparative Methods Review.Molecular & cellular proteomics : MCP · 2022Review
- Progress of CRISPR-Cas13 Mediated Live-Cell RNA Imaging and Detection of RNA-Protein Interactions.Frontiers in cell and developmental biology · 2022Review
- Poly(A)+ Sensing of Hybridization-Sensitive Fluorescent Oligonucleotide Probe Characterized by Fluorescence Correlation Methods.International journal of molecular sciences · 2021Article
- Broad Applications of Thiazole Orange in Fluorescent Sensing of Biomolecules and Ions.Molecules (Basel, Switzerland) · 2021Review
- DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.Advances in experimental medicine and biology · 2021Article
- Oligonucleotide Probe for TranscriptomeACS chemical biology · 2020Article
- Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression.Annual review of biochemistry · 2020Review
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- RNA imaging in living mice enabled by anChemical science · 2020Article
- Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids.Applied microscopy · 2019Review
- Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Design of thiazole orange oligonucleotide probes for detection of DNA and RNA by fluorescence and duplex melting.Organic & biomolecular chemistry · 2019Article
- RNA mGenome biology · 2018Article
- Fluorescent turn-on probes for wash-free mRNA imagingChemical science · 2017Article
- Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes.Chemical Society reviews · 2017Review
- Combination probes with intercalating anchors and proximal fluorophores for DNA and RNA detection.Nucleic acids research · 2016Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elucidating the dynamic organization of nuclear RNA foci is important for understanding and manipulating these functional sites of gene expression in both physiological and pathological states. However, such studies have been difficult to establish in vivo as a result of the absence of suitable RNA imaging methods. Here, we describe a high-resolution fluorescence RNA imaging method, ECHO-liveFISH, to label endogenous nuclear RNA in living mice and chicks. Upon in vivo electroporation, exciton-controlled sequence-specific oligonucleotide probes revealed focally concentrated endogenous 28S rRNA and U3 snoRNA at nucleoli and poly(A) RNA at nuclear speckles. Time-lapse imaging reveals steady-state stability of these RNA foci and dynamic dissipation of 28S rRNA concentrations upon polymerase I inhibition in native brain tissue. Confirming the validity of this technique in a physiological context, the in vivo RNA labeling did not interfere with the function of target RNA nor cause noticeable cytotoxicity or perturbation of cellular behavior.
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