ArticleNucleic acids research2015
Post-translational environmental switch of RadA activity by extein-intein interactions in protein splicing.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 58 citations in OpenAlex.
- A protein chaperone can stabilize the intein-containing precursor to indirectly promote protein splicing.Protein science : a publication of the Protein Society · 2026Article
- Intein splicing efficiency and RadA levels can control the mode of archaeal DNA replication.Science advances · 2024Article
- Conditional protein splicing of the Mycobacterium tuberculosis RecA intein in its native host.Scientific reports · 2024Article
- Article
- Conditional protein splicing of thebioRxiv : the preprint server for biology · 2024Article
- An intein-based biosensor to measure protein stability in vivo.Protein science : a publication of the Protein Society · 2024Article
- Neighboring inteins interfere with one another's homing capacity.PNAS nexus · 2023Article
- Inteins-mechanism of protein splicing, emerging regulatory roles, and applications in protein engineering.Frontiers in microbiology · 2023Review
- Article
- SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein histidines.Bioscience reports · 2022Article
- Conditional Alternative Protein Splicing Promoted by Inteins fromBiochemistry · 2022Article
- Reactive Chlorine Species Reversibly Inhibit DnaB Protein Splicing in Mycobacteria.Microbiology spectrum · 2021Article
- Monitoring Protein Splicing Using In-gel Fluorescence Immediately Following SDS-PAGE.Bio-protocol · 2021Article
- Mini-Intein Structures from Extremophiles Suggest a Strategy for Finding Novel Robust Inteins.Microorganisms · 2021Article
- Construction and Quantitation of a Selectable Protein Splicing Sensor Using Gibson Assembly and Spot Titers.Current protocols · 2021Article
- Intein Inhibitors as Novel Antimicrobials: Protein Splicing in Human Pathogens, Screening Methods, and Off-Target Considerations.Frontiers in molecular biosciences · 2021Review
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- Protein Splicing Activity of theBiochemistry · 2020Article
- Article
- Allosteric Influence of Extremophile Hairpin Motif Mutations on the Protein Splicing Activity of a Hyperthermophilic Intein.Biochemistry · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Post-translational control based on an environmentally sensitive intervening intein sequence is described. Inteins are invasive genetic elements that self-splice at the protein level from the flanking host protein, the exteins. Here we show in Escherichia coli and in vitro that splicing of the RadA intein located in the ATPase domain of the hyperthermophilic archaeon Pyrococcus horikoshii is strongly regulated by the native exteins, which lock the intein in an inactive state. High temperature or solution conditions can unlock the intein for full activity, as can remote extein point mutations. Notably, this splicing trap occurs through interactions between distant residues in the native exteins and the intein, in three-dimensional space. The exteins might thereby serve as an environmental sensor, releasing the intein for full activity only at optimal growth conditions for the native organism, while sparing ATP consumption under conditions of cold-shock. This partnership between the intein and its exteins, which implies coevolution of the parasitic intein and its host protein may provide a novel means of post-translational control.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.