ArticleDevelopmental biology2015
Molecular asymmetry in the 8-cell stage Xenopus tropicalis embryo described by single blastomere transcript sequencing.
Article in Developmental biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- A maternal dorsoventral prepattern revealed by an asymmetric distribution of ventralizing molecules before fertilization inFrontiers in cell and developmental biology · 2024Article
- RNA localization during early development of the axolotl.Frontiers in cell and developmental biology · 2023Article
- Maternal Wnt11b regulates cortical rotation during Xenopus axis formation: analysis of maternal-effect wnt11b mutants.Development (Cambridge, England) · 2022Article
- High-Throughput, Comprehensive Single-Cell Proteomic Analysis ofAnalytical chemistry · 2022Article
- In Vivo Subcellular Mass Spectrometry Enables Proteo-Metabolomic Single-Cell Systems Biology in a Chordate Embryo Developing to a Normally Behaving Tadpole (X. laevis)*.Angewandte Chemie (International ed. in English) · 2021Article
- Altering metabolite distribution at Xenopus cleavage stages affects left-right gene expression asymmetries.Genesis (New York, N.Y. : 2000) · 2021Article
- Chondrodysplasias With Multiple Dislocations Caused by Defects in Glycosaminoglycan Synthesis.Frontiers in genetics · 2021Review
- Transcriptomics and Proteomics Methods forCold Spring Harbor protocols · 2020Article
- Early Xenopus gene regulatory programs, chromatin states, and the role of maternal transcription factors.Current topics in developmental biology · 2020Review
- Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals.Nature communications · 2019Article
- Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation.Cell reports · 2019Article
- Cap-Independent mRNA Translation in Germ Cells.International journal of molecular sciences · 2019Review
- Transcriptomics of dorso-ventral axis determination in Xenopus tropicalis.Developmental biology · 2018Article
- Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan.Scientific reports · 2018Article
- Metabolic Comparison of DorsalAnalytical methods : advancing methods and applications · 2017Article
- A gene regulatory program controlling early Xenopus mesendoderm formation: Network conservation and motifs.Seminars in cell & developmental biology · 2017Review
- Spemann organizer transcriptome induction by early beta-catenin, Wnt, Nodal, and Siamois signals inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- High-throughput analysis reveals novel maternal germline RNAs crucial for primordial germ cell preservation and proper migration.Development (Cambridge, England) · 2017Article
- Maternal messages to live by: a personal historical perspective.Genesis (New York, N.Y. : 2000) · 2017Review
- Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using Capillary Electrophoresis Electrospray Ionization High-Resolution Mass Spectrometry (CE-ESI-HRMS).Molecular & cellular proteomics : MCP · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Correct development of the vertebrate body plan requires the early definition of two asymmetric, perpendicular axes. The first axis is established during oocyte maturation, and the second is established by symmetry breaking shortly after fertilization. The physical processes generating the second asymmetric, or dorsal-ventral, axis are well understood, but the specific molecular determinants, presumed to be maternal gene products, are poorly characterized. Whilst enrichment of maternal mRNAs at the animal and vegetal poles in both the oocyte and the early embryo has been studied, little is known about the distribution of maternal mRNAs along either the dorsal-ventral or left-right axes during the early cleavage stages. Here we report an unbiased analysis of the distribution of maternal mRNA on all axes of the Xenopus tropicalis 8-cell stage embryo, based on sequencing of single blastomeres whose positions within the embryo are known. Analysis of pooled data from complete sets of blastomeres from four embryos has identified 908 mRNAs enriched in either the animal or vegetal blastomeres, of which 793 are not previously reported as enriched. In contrast, we find no evidence for asymmetric distribution along either the dorsal-ventral or left-right axes. We confirm that animal pole enrichment is on average distinctly lower than vegetal pole enrichment, and that considerable variation is found between reported enrichment levels in different studies. We use publicly available data to show that there is a significant association between genes with human disease annotation and enrichment at the animal pole. Mutations in the human ortholog of the most animally enriched novel gene, Slc35d1, are causative for Schneckenbecken dysplasia, and we show that a similar phenotype is produced by depletion of the orthologous protein in Xenopus embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.