ArticleProceedings of the National Academy of Sciences of the United States of America2015
A structural, functional, and computational analysis suggests pore flexibility as the base for the poor selectivity of CNG channels.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Atomistic mechanism of non-selective cation permeation in cyclic nucleotide-gated CNGA1 ion channel by molecular dynamics simulations.Communications biology · 2025Article
- Structure-function analysis of CNGA3-associated achromatopsia patient variants complements clinical genomics in pathogenicity determination.Orphanet journal of rare diseases · 2025Article
- Structural insights into ion selectivity and transport mechanisms of Oryza sativa HKT2;1 and HKT2;2/1 transporters.Nature plants · 2024Article
- Collective Domain Motion Facilitates Water Transport in SGLT1.International journal of molecular sciences · 2023Article
- Mechanism of Calcium Permeation in a Glutamate Receptor Ion Channel.Journal of chemical information and modeling · 2023Article
- Molecular Events behind the Selectivity and Inactivation Properties of Model NaK-Derived Ion Channels.International journal of molecular sciences · 2022Article
- Ion behavior in the selectivity filter of HCN1 channels.Biophysical journal · 2022Article
- The conformational plasticity of the selectivity filter methionines controls the in-cell Cu(I) uptake through the CTR1 transporter.QRB discovery · 2022Article
- Ion-dependent structure, dynamics, and allosteric coupling in a non-selective cation channel.Nature communications · 2021Article
- CNG channel structure, function, and gating: a tale of conformational flexibility.Pflugers Archiv : European journal of physiology · 2021Review
- Gating movements and ion permeation in HCN4 pacemaker channels.Molecular cell · 2021Article
- Nonselective cation permeation in an AMPA-type glutamate receptor.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Dynamic interaction of fluoroquinolones with magnesium ions monitored using bacterial outer membrane nanopores.Chemical science · 2020Article
- Voltage vs. Ligand I: Structural basis of the intrinsic flexibility of S3 segment and its significance in ion channel activation.Channels (Austin, Tex.) · 2019Article
- The permeation mechanism of organic cations through a CNG mimic channel.PLoS computational biology · 2018Article
- Site-specific ion occupation in the selectivity filter causes voltage-dependent gating in a viral KScientific reports · 2018Article
- Identification of essential amino acids for glucose transporter 5 (GLUT5)-mediated fructose transport.The Journal of biological chemistry · 2018Article
- Structural insights into the mechanisms of CNBD channel function.The Journal of general physiology · 2018Review
- The gating mechanism in cyclic nucleotide-gated ion channels.Scientific reports · 2018Article
- New views on phototransduction from atomic force microscopy and single molecule force spectroscopy on native rods.Scientific reports · 2017Article
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12 authors.
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Abstract
Cyclic nucleotide-gated (CNG) ion channels, despite a significant homology with the highly selective K(+) channels, do not discriminate among monovalent alkali cations and are permeable also to several organic cations. We combined electrophysiology, molecular dynamics (MD) simulations, and X-ray crystallography to demonstrate that the pore of CNG channels is highly flexible. When a CNG mimic is crystallized in the presence of a variety of monovalent cations, including Na(+), Cs(+), and dimethylammonium (DMA(+)), the side chain of Glu66 in the selectivity filter shows multiple conformations and the diameter of the pore changes significantly. MD simulations indicate that Glu66 and the prolines in the outer vestibule undergo large fluctuations, which are modulated by the ionic species and the voltage. This flexibility underlies the coupling between gating and permeation and the poor ionic selectivity of CNG channels.
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