ArticleJournal of medical virology2016
Benefits and drawbacks of molecular techniques for diagnosis of viral respiratory infections. Experience with two multiplex PCR assays.
Article in Journal of medical virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Rapid Bacteria Identification and Antimicrobial Susceptibility Testing via 5-Min Fluorescence In Situ Hybridization.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Human metapneumovirus (hMPV) in 2025: emerging trends and insights from community and hospital-based respiratory panel analyses-a comprehensive review.Virology journal · 2025Review
- Machine learning in point-of-care testing: innovations, challenges, and opportunities.Nature communications · 2025Review
- Proteomic profiling of the local and systemic immune response to pediatric respiratory viral infections.mSystems · 2025Article
- Clinical Presentation and Co-Detection of Respiratory Pathogens in Children Under 5 Years with Non-COVID-19 Bacterial and Viral Respiratory Tract Infections: A Prospective Study in Białystok, Poland (2021-2022).Medical science monitor : international medical journal of experimental and clinical research · 2023Article
- Prevalence and seasonality of viral respiratory infections in a temperate climate region: A 24-year study (1997-2020).Influenza and other respiratory viruses · 2022Article
- SARS-CoV-2 in hospital air as revealed by comprehensive respiratory viral panel sequencing.Infection prevention in practice · 2022Article
- Flow Cytometry: From Experimental Design to Its Application in the Diagnosis and Monitoring of Respiratory Diseases.International journal of molecular sciences · 2020Review
- Enteric Virome and Carcinogenesis in the Gut.Digestive diseases and sciences · 2020Review
- Role of multiplex PCR analysis in children with febrile seizures.Wiener medizinische Wochenschrift (1946) · 2017Article
- Diagnostic yield and clinical impact of routine cell culture for respiratory viruses among children with a negative multiplex RT-PCR result.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2017Article
- Dual RNA-seq reveals viral infections in asthmatic children without respiratory illness which are associated with changes in the airway transcriptome.Genome biology · 2017Article
- Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).Scientific reports · 2016Article
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Authors and funding
6 authors.
Funding
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Abstract
Molecular techniques have represented a major step forward in the diagnosis of viral respiratory infections. They are considered highly sensitive and specific compared to conventional techniques. In this study two nucleic acid amplification tests (NAATs) were compared to conventional methods (immunofluorescence and viral culture). The aim of this work was to discuss the clinical interpretation of the results obtained by NAATs on the basis of the two-decade experience of our group and the literature. Eighty nasopharyngeal aspirates were collected from children under six years attended for acute respiratory illness at the pediatric emergency room of a third level Hospital. Both NAATs tested (Seeplex(®) and Clart(®)) showed an overall higher performance regarding sensitivity (76% and 90%, respectively). Compared to Seeplex(®), the Clart(®) system tripled the number of multiple detections (8 by Seeplex(®) vs. 25 by Clart(®)). In some specimens both NAATs detected different viruses. Given these discrepancies and the fact that detection of viral nucleic acids is not necessarily related to the current clinical syndrome, the interpretation of molecular results may not always be so straightforward. The pros and cons of NAATs should always be taken into account when giving a result.
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