ArticlePloS one2015
Next Generation Sequencing and Transcriptome Analysis Predicts Biosynthetic Pathway of Sennosides from Senna (Cassia angustifolia Vahl.), a Non-Model Plant with Potent Laxative Properties.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 90 citations in OpenAlex.
- Functional characterization of two distinct classes of NADPH-cytochrome P450 reductases in Senna alexandrina Mill.Molecular biology reports · 2025Article
- Sennoside A represses the malignant phenotype and tumor immune microenvironment of non-small cell lung cancer cells by inhibiting the TRAF6/NF-κB pathway.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L.Scientific reports · 2025Article
- Unveiling Anthraquinones: Diverse Health Benefits of an Essential Secondary Metabolite.Recent patents on biotechnology · 2025Review
- Comparative transcriptome analysis and HPLC reveal candidate genes associated with synthesis of bioactive constituents inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Enriched soil amendments influenced soil fertility, herbage yield and bioactive principle of medicinal plant (Heliyon · 2024Article
- Development of highly discriminatory SCoT- and CBDP-based SCAR fingerprint for authentication of Indian senna (Frontiers in plant science · 2024Article
- Comparative LC-ESIMS-Based Metabolite Profiling ofMetabolites · 2023Article
- Comparative Transcriptomic Analysis of Genes in the 20-Hydroxyecdysone Biosynthesis in the FernInternational journal of molecular sciences · 2023Article
- Comparative Transcriptome Analyses of DifferentGenes · 2022Article
- De Novo Transcriptome Analysis Reveals Putative Genes Involved in Anthraquinone Biosynthesis inGenes · 2022Article
- Transcriptome and HPLC Analysis Reveal the Regulatory Mechanisms of Aurantio-Obtusin in Space Environment-InducedInternational journal of environmental research and public health · 2022Article
- Comparative transcriptome analysis of different tissues of Rheum tanguticum Maxim. ex Balf. (Polygonaceae) reveals putative genes involved in anthraquinone biosynthesis.Genetics and molecular biology · 2022Article
- Transcriptome profiling and differential gene expression analysis provides insights into3 Biotech · 2021Article
- Tissue-specific transcriptome forPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Review
- Tissue-specific transcriptome analyses reveal candidate genes for stilbene, flavonoid and anthraquinone biosynthesis in the medicinal plant Polygonum cuspidatum.BMC genomics · 2021Article
- Article
- Transcriptome analysis for molecular landscaping of genes controlling diterpene andrographolide biosynthesis in3 Biotech · 2020Article
- Integrated Analysis of Transcriptome and Metabolome inACS omega · 2020Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Senna (Cassia angustifolia Vahl.) is a world's natural laxative medicinal plant. Laxative properties are due to sennosides (anthraquinone glycosides) natural products. However, little genetic information is available for this species, especially concerning the biosynthetic pathways of sennosides. We present here the transcriptome sequencing of young and mature leaf tissue of Cassia angustifolia using Illumina MiSeq platform that resulted in a total of 6.34 Gb of raw nucleotide sequence. The sequence assembly resulted in 42230 and 37174 transcripts with an average length of 1119 bp and 1467 bp for young and mature leaf, respectively. The transcripts were annotated using NCBI BLAST with 'green plant database (txid 33090)', Swiss Prot, Kyoto Encylcopedia of Genes & Genomes (KEGG), Cluster of Orthologous Gene (COG) and Gene Ontology (GO). Out of the total transcripts, 40138 (95.0%) and 36349 (97.7%) from young and mature leaf, respectively, were annotated by BLASTX against green plant database of NCBI. We used InterProscan to see protein similarity at domain level, a total of 34031 (young leaf) and 32077 (mature leaf) transcripts were annotated against the Pfam domains. All transcripts from young and mature leaf were assigned to 191 KEGG pathways. There were 166 and 159 CDS, respectively, from young and mature leaf involved in metabolism of terpenoids and polyketides. Many CDS encoding enzymes leading to biosynthesis of sennosides were identified. A total of 10,763 CDS differentially expressing in both young and mature leaf libraries of which 2,343 (21.7%) CDS were up-regulated in young compared to mature leaf. Several differentially expressed genes found functionally associated with sennoside biosynthesis. CDS encoding for many CYPs and TF families were identified having probable roles in metabolism of primary as well as secondary metabolites. We developed SSR markers for molecular breeding of senna. We have identified a set of putative genes involved in various secondary metabolite pathways, especially those related to the synthesis of sennosides which will serve as an important platform for public information about gene expression, genomics, and functional genomics in senna.
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