ArticleBiomacromolecules2015
Covalent Modification of Synthetic Hydrogels with Bioactive Proteins via Sortase-Mediated Ligation.
Article in Biomacromolecules, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 2 citations in OpenAlex.
- Enzymatic Methods for Assembling and Modifying Hydrogel Biomaterials.Regenerative engineering and translational medicine · 2025Article
- Molecular Engineering of Recombinant Protein Hydrogels: Programmable Design and Biomedical Applications.Gels (Basel, Switzerland) · 2025Review
- Bioorthogonal Engineering of Cellular Microenvironments Using Isonitrile Ligations.Advanced functional materials · 2025Article
- Engineered Protein Hydrogels as Biomimetic Cellular Scaffolds.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Conjugation of IL-33 to Microporous Annealed Particle Scaffolds Enhances Type 2-Like Immune Responses In Vitro and In Vivo.Advanced healthcare materials · 2024Article
- Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Chemical and Biological Engineering Strategies to Make and Modify Next-Generation Hydrogel Biomaterials.Matter · 2023Article
- Organoid co-culture model of the human endometrium in a fully synthetic extracellular matrix enables the study of epithelial-stromal crosstalk.Med (New York, N.Y.) · 2023Article
- Accelerating aging with dynamic biomaterials: Recapitulating aged tissue phenotypes in engineered platforms.iScience · 2023Review
- Research Progress in Enzymatically Cross-Linked Hydrogels as Injectable Systems for Bioprinting and Tissue Engineering.Gels (Basel, Switzerland) · 2023Review
- Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering.Frontiers in bioengineering and biotechnology · 2023Article
- Chemical strategies to engineer hydrogels for cell culture.Nature reviews. Chemistry · 2022Review
- Elastic Properties of Poly(ethylene glycol) Nanomembranes and Respective Implications.Membranes · 2022Article
- Vitronectin-based hydrogels recapitulate neuroblastoma growth conditions.Frontiers in cell and developmental biology · 2022Article
- Sequestered cell-secreted extracellular matrix proteins improve murine folliculogenesis and oocyte maturation for fertility preservation.Acta biomaterialia · 2021Article
- Click-functionalized hydrogel design for mechanobiology investigations.Molecular systems design & engineering · 2021Article
- Encapsulation of murine hematopoietic stem and progenitor cells in a thiol-crosslinked maleimide-functionalized gelatin hydrogel.Acta biomaterialia · 2021Article
- Recent advancements in enzyme-mediated crosslinkable hydrogels:APL bioengineering · 2021Review
- Vascularized organoids on a chip: strategies for engineering organoids with functional vasculature.Lab on a chip · 2021Review
- Dynamic Click Hydrogels for Xeno-Free Culture of Induced Pluripotent Stem Cells.Advanced biosystems · 2020Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Synthetic extracellular matrices are widely used in regenerative medicine and as tools in building in vitro physiological culture models. Synthetic hydrogels display advantageous physical properties, but are challenging to modify with large peptides or proteins. Here, a facile, mild enzymatic postgrafting approach is presented. Sortase-mediated ligation was used to conjugate human epidermal growth factor fused to a GGG ligation motif (GGG-EGF) to poly(ethylene glycol) (PEG) hydrogels containing the sortase LPRTG substrate. The reversibility of the sortase reaction was then exploited to cleave tethered EGF from the hydrogels for analysis. Analyses of the reaction supernatant and the postligation hydrogels showed that the amount of tethered EGF increases with increasing LPRTG in the hydrogel or GGG-EGF in the supernatant. Sortase-tethered EGF was biologically active, as demonstrated by stimulation of DNA synthesis in primary human hepatocytes and endometrial epithelial cells. The simplicity, specificity, and reversibility of sortase-mediated ligation and cleavage reactions make it an attractive approach for modification of hydrogels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.