ArticlePloS one2015
Alleviating Redox Imbalance Enhances 7-Dehydrocholesterol Production in Engineered Saccharomyces cerevisiae.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 45 citations in OpenAlex.
- Metabolic Engineering of Yeasts for the Production of the Triterpene Squalene: Current Status and Future Prospective.Microorganisms · 2025Review
- Combinatorial metabolic engineering ofEngineering microbiology · 2023Article
- Bottom-up synthetic biology approach for improving the efficiency of menaquinone-7 synthesis in Bacillus subtilis.Microbial cell factories · 2022Article
- Recent progress in strategies for steroid production in yeasts.World journal of microbiology & biotechnology · 2022Review
- Reengineering of 7-dehydrocholesterol biosynthesis inFrontiers in microbiology · 2022Article
- Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast.Bioresources and bioprocessing · 2021Review
- Compartmentalized Reconstitution of Post-Frontiers in microbiology · 2021Article
- Fermentative production of Vitamin E tocotrienols in Saccharomyces cerevisiae under cold-shock-triggered temperature control.Nature communications · 2020Article
- Yeast as a promising heterologous host for steroid bioproduction.Journal of industrial microbiology & biotechnology · 2020Review
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2020Article
- Primary and Secondary Metabolic Effects of a Key Gene Deletion (ΔApplied and environmental microbiology · 2019Article
- Rate-limiting steps in the Saccharomyces cerevisiae ergosterol pathway: towards improved ergosta-5,7-dien-3β-ol accumulation by metabolic engineering.World journal of microbiology & biotechnology · 2018Article
- Metabolic engineering ofBiotechnology for biofuels · 2018Article
- Cell foundry with high product specificity and catalytic activity for 21-deoxycortisol biotransformation.Microbial cell factories · 2017Article
- Redox potential driven aeration during very-high-gravity ethanol fermentation by using flocculating yeast.Scientific reports · 2016Article
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6 authors at 2 institutions in 1 country.
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Abstract
Maintaining redox balance is critical for the production of heterologous secondary metabolites, whereas on various occasions the native cofactor balance does not match the needs in engineered microorganisms. In this study, 7-dehydrocholesterol (7-DHC, a crucial precursor of vitamin D3) biosynthesis pathway was constructed in Saccharomyces cerevisiae BY4742 with endogenous ergosterol synthesis pathway blocked by knocking out the erg5 gene (encoding C-22 desaturase). The deletion of erg5 led to redox imbalance with higher ratio of cytosolic free NADH/NAD+ and more glycerol and ethanol accumulation. To alleviate the redox imbalance, a water-forming NADH oxidase (NOX) and an alternative oxidase (AOX1) were employed in our system based on cofactor regeneration strategy. Consequently, the production of 7-dehydrocholesterol was increased by 74.4% in shake flask culture. In the meanwhile, the ratio of free NADH/NAD+ and the concentration of glycerol and ethanol were reduced by 78.0%, 50.7% and 7.9% respectively. In a 5-L bioreactor, the optimal production of 7-DHC reached 44.49(±9.63) mg/L. This study provides a reference to increase the production of some desired compounds that are restricted by redox imbalance.
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