ArticleTransfusion2015
Renitrosylation of banked human red blood cells improves deformability and reduces adhesivity.
Article in Transfusion, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- An on-chip deformability checker demonstrates that the severity of iron deficiency is associated with increased deformability of red blood cells.Scientific reports · 2025Article
- Modulation of the allosteric and vasoregulatory arms of erythrocytic oxygen transport.Frontiers in physiology · 2024Review
- Flickering of fetal erythrocytes membrane under gestational diabetes observed with dual time resolved membrane fluctuation spectroscopy.Biochemistry and biophysics reports · 2023Article
- Generation and Export of Red Blood Cell ATP in Health and Disease.Frontiers in physiology · 2021Review
- Role of Nitric Oxide Carried by Hemoglobin in Cardiovascular Physiology: Developments on a Three-Gas Respiratory Cycle.Circulation research · 2020Review
- Transfusion in children with acute respiratory distress syndrome.Annals of translational medicine · 2019Review
- Nitric oxide loading reduces sickle red cell adhesion and vaso-occlusion in vivo.Blood advances · 2019Article
- Protective Effect of Dinitrosyl Iron Complexes with Glutathione in Red Blood Cell Lysis Induced by Hypochlorous Acid.Oxidative medicine and cellular longevity · 2019Article
- Red Blood Cell Deformability, Vasoactive Mediators, and Adhesion.Frontiers in physiology · 2019Review
- S-Nitrosohemoglobin Levels and Patient Outcome After Transfusion During Pediatric Bypass Surgery.Clinical and translational science · 2018Observational
- On the Effects of Reactive Oxygen Species and Nitric Oxide on Red Blood Cell Deformability.Frontiers in physiology · 2018Article
- Transfusion of Red Blood Cells to Patients with Sepsis.International journal of molecular sciences · 2017Review
- Antagonists of the system L neutral amino acid transporter (LAT) promote endothelial adhesivity of human red blood cells.Thrombosis and haemostasis · 2017Article
- Red blood cell storage time and transfusion: current practice, concerns and future perspectives.Blood transfusion = Trasfusione del sangue · 2017Review
- Effects of storage duration of suspended red blood cells before intraoperative infusion on coagulation indexes, routine blood examination and immune function in patients with gastrointestinal tumors.Pakistan journal of medical sciencesArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundTransfusion of red blood cells (RBCs) is a frequent health care practice. However, unfavorable consequences may occur from transfusions of stored RBCs and are associated with RBC changes during storage. Loss of S-nitrosohemoglobin (SNO-Hb) and other S-nitrosothiols (SNOs) during storage is implicated as a detriment to transfusion efficacy. It was hypothesized that restoring SNOs within banked RBCs would improve RBC functions relevant to successful transfusion outcomes, namely, increased deformability and decreased adhesivity. STUDY DESIGN AND
methodsStored human RBCs were incubated with nitric oxide (NO) donors PROLI/NO and DEA/NO (disodium 1-[2-(carboxylato)-pyrrolidin-1-yl]diazen-1-ium-1,2-diolate and diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate) under varying experimental conditions (e.g., aerobic/anaerobic incubation, NO donor to RBC ratio). SNO restoration was evaluated in vitro and in vivo as a means to improve RBC function after storage.
resultsIncubation of RBCs with the NO donors resulted in 10-fold greater levels of SNO-Hb versus untreated control or sham RBCs, with significantly higher Hb-bound NO yields from an NO dose delivered by DEA/NO. RBC incubation with DEA/NO at a stoichiometry of 1:62.5 NO:Hb significantly increased RBC deformabilty and reduced adhesion to cultured endothelial cells. RBC incubation with DEA/NO also increased S-nitrosylation of RBC cytoskeletal and membrane proteins, including the β-spectrin chain. Renitrosylation attenuated both RBC sequestration in the lung and the mild blood oxygen saturation impairments seen with banked RBCs in a mouse model of transfusion.
conclusionsRBC renitrosylation using NO donors has promise for correcting deficient properties (e.g., adhesivity, rigidity, and SNO loss) of banked RBCs and in turn improving transfusion outcomes.
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