ArticleNature methods2015
Combining protein and mRNA quantification to decipher transcriptional regulation.
Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 145 citations in OpenAlex.
- Deep learning-based high-resolution time inference for deciphering dynamic gene regulation from fixed embryos.Nature communications · 2025Article
- The human intelligence evolved from proximalQuantitative biology (Beijing, China) · 2025Article
- Commentary to "The human intelligence evolved from proximalQuantitative biology (Beijing, China) · 2025Article
- Single-Cell Multiomic Analysis of Circadian Rhythmicity in Mouse Liver.bioRxiv : the preprint server for biology · 2025Article
- Visualization of the Cytoskeleton in Fixed Drosophila Embryos.Current protocols · 2025Article
- Uncovering dynamic transcriptional regulation of methanogenesis via single-cell imaging of archaeal gene expression.Nature communications · 2025Article
- MONITTR allows real-time imaging of transcription and endogenous proteins in C. elegans.The Journal of cell biology · 2025Article
- Transcription factor clusters as information transfer agents.Science advances · 2025Article
- Reducing Cofilin dosage makes embryos resilient to heat stress.bioRxiv : the preprint server for biology · 2025Article
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- A generative benchmark for evaluating the performance of fluorescent cell image segmentation.Synthetic and systems biotechnology · 2024Article
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- Optogenetic dissection of transcriptional repression in a multicellular organism.Nature communications · 2024Article
- How Transcription Factor Clusters Shape the Transcriptional Landscape.Biomolecules · 2024Review
- Unified bursting strategies in ectopic and endogenousbioRxiv : the preprint server for biology · 2024Article
- Transcriptional bursting dynamics in gene expression.Frontiers in genetics · 2024Review
- Biophysical modeling with variational autoencoders for bimodal, single-cell RNA sequencing data.bioRxiv : the preprint server for biology · 2023Article
- Size limits the sensitivity of kinetic schemes.Nature communications · 2023Article
- Competing constraints shape the nonequilibrium limits of cellular decision-making.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Gene expression model inference from snapshot RNA data using Bayesian non-parametrics.Nature computational science · 2023Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We combine immunofluorescence and single-molecule fluorescence in situ hybridization (smFISH), followed by automated image analysis, to quantify the concentration of nuclear transcription factors, number of transcription factors bound, and number of nascent mRNAs synthesized at individual gene loci. A theoretical model is used to decipher how transcription factor binding modulates the stochastic kinetics of mRNA production. We demonstrate this approach by examining the regulation of hunchback in the early Drosophila embryo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.