ArticleNature methods2015
A naturally monomeric infrared fluorescent protein for protein labeling in vivo.
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Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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89citing papers in PubMed
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Who cites it
89 citing papers in PubMed.
- ILK-Dependent Modulation of DPP4 Prevents Progression of Calcific Aortic Valve Disease.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- Protocol for stable cell line production to express muscle-type nicotinic receptor.STAR protocols · 2026Article
- Asymmetric division in a two-cell-like state rejuvenates embryonic stem cells.Cell research · 2026Article
- Glycerol 3-phosphate acyltransferase exacerbates α-synuclein-induced toxicity by increasing lipid peroxidation.Nature communications · 2026Article
- Smoothened turnover regulated by Hedgehog signaling in Drosophila.bioRxiv : the preprint server for biology · 2026Article
- The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- TDP-43 skein-like inclusions are formed by BAG3- and HSP70-guided co-aggregation with actin-binding proteins.Nature cell biology · 2025Article
- Far-red fluorescent genetically encoded calcium ion indicators.Nature communications · 2025Article
- Calmodulin regulates TRPV5 intracellular trafficking and plasma membrane abundance.The Journal of physiology · 2024Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- MYC phase separation selectively modulates the transcriptome.Nature structural & molecular biology · 2024Article
- Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag.Nature communications · 2024Article
- Development of an miRFP680-Based Fluorescent Calcium Ion Biosensor Using End-Optimized Transposons.ACS sensors · 2024Article
- Let it glow: genetically encoded fluorescent reporters in Plasmodium.Malaria journal · 2024Review
- Phase separation of YAP-MAML2 differentially regulates the transcriptome.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Model-Based Explainable Deep Learning for Light-Field Microscopy Imaging.IEEE transactions on image processing : a publication of the IEEE Signal Processing Society · 2024Article
- Rewarding Capacity of Optogenetically Activating a Giant GABAergic Central-Brain Interneuron in LarvalThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Quantitative assessment of near-infrared fluorescent proteins.Nature methods · 2023Article
- Current Trends of Bacterial and Fungal Optoproteins for Novel Optical Applications.International journal of molecular sciences · 2023Review
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
16 authors.
Dan Yu1] Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA. [2] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.ORCID 0000000223176696
Michelle A Baird1] National High Magnetic Field Laboratory, The Florida State University, Tallahassee, Florida, USA. [2] Department of Biological Science, The Florida State University, Tallahassee, Florida, USA.
John R Allen1] National High Magnetic Field Laboratory, The Florida State University, Tallahassee, Florida, USA. [2] Department of Biological Science, The Florida State University, Tallahassee, Florida, USA.
Elizabeth S Howe1] National High Magnetic Field Laboratory, The Florida State University, Tallahassee, Florida, USA. [2] Department of Biological Science, The Florida State University, Tallahassee, Florida, USA.
Matthew P Klassen1] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA. [2] Department of Physiology, University of California, San Francisco, San Francisco, California, USA. [3] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA.
Anna ReadeCardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Kalpana Makhijani1] Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA. [2] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Yuanquan Song1] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA. [2] Department of Physiology, University of California, San Francisco, San Francisco, California, USA. [3] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA.
Songmei LiuCardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Zehra MurthyCardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Shao-Qing Zhang1] Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA. [2] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA. [3] Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Orion D WeinerCardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Thomas B KornbergCardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Yuh-Nung Jan1] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA. [2] Department of Physiology, University of California, San Francisco, San Francisco, California, USA. [3] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA.
Michael W Davidson1] National High Magnetic Field Laboratory, The Florida State University, Tallahassee, Florida, USA. [2] Department of Biological Science, The Florida State University, Tallahassee, Florida, USA.
Xiaokun Shu1] Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA. [2] Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Funding
Cellular Decision Making Diversity SupplementR35GM118167 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Orion D Weiner · 2016 to 2026
$10.3MPREDOCTORAL TRAINING IN DEVELOPMENTAL BIOLOGYT32HD007470 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Todd Nystul · 1994 to 2026
$7.7MMOLECULAR MECHANISMS IN DEVELOPMENTR01GM030637 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KORNBERG, THOMAS B. · 1985 to 2017
$6.8MDe novo design of alpha-helical bundlesR37GM054616 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI DEGRADO, WILLIAM · 2004 to 2013
$3.6MSignal Integration in Neutrophil ChemotaxisR01GM084040 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WEINER, ORION D · 2008 to 2015
$2.6MDE NOVO DESIGN OF ALPHA HELICAL BUNDLESR01GM054616 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI DEGRADO, WILLIAM · 1996 to 2016
$2.4MA Toolkit for Light-Control of Molecular Processes in Living CellsR01GM096164 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI VOIGT, CHRISTOPHER A, WEINER, ORION D · 2012 to 2015
$1.8MHoward Hughes Medical InstituteNICHD NIH HHS T32 HD007470NIGMS NIH HHS GM030637NIGMS NIH HHS GM054616NIGMS NIH HHS GM084040NIGMS NIH HHS GM096164NIGMS NIH HHS R01 GM030637NIGMS NIH HHS R01 GM054616NIGMS NIH HHS R01 GM084040NIGMS NIH HHS R01 GM096164NIGMS NIH HHS R35 GM118167NIGMS NIH HHS R37 GM054616
Abstract
Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology.
Indexed as
Infrared RaysAmino Acid SequenceAnimalsAnimals, Genetically ModifiedDevelopmental BiologyDNADrosophila melanogasterFluorescent DyesGreen Fluorescent ProteinsHeLa CellsHistidineHumansLuminescent ProteinsMiceMolecular Sequence DataMutationDNAFluorescent DyesGreen Fluorescent ProteinsHistidineLuminescent ProteinspolyhistidineRecombinant Fusion Proteins
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