ArticleThe arabidopsis book2015
Co-Immunoprecipitation of Membrane-Bound Receptors.
Article in The arabidopsis book, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Ligand-Baited Proteomics Reveals Vesicle Trafficking Proteins as Putative Site of Action of Indaziflam.Journal of agricultural and food chemistry · 2026Article
- IMK2-IMK3 module regulates biogenesis of nascent cell walls and postcytokinetic differentiation inScience advances · 2026Article
- Comparative proteomic profiling of receptor kinase signaling reveals key trafficking components enforcing plant stomatal development.Science advances · 2026Article
- Mass Spectrometry Proteomics: A Key to Faster Drug Discovery.Journal of medicinal chemistry · 2026Review
- Proteomic analysis of the pyrenoid-traversing membranes of Chlamydomonas reinhardtii reveals novel components.The New phytologist · 2026Article
- Proteomic analysis of the pyrenoid-traversing membranes ofbioRxiv : the preprint server for biology · 2025Article
- Comparative Proteomic Profiling of Receptor Kinase Signaling Reveals Key Trafficking Components Enforcing Plant Stomatal Development.bioRxiv : the preprint server for biology · 2025Article
- Heterodimerization of Chemoreceptors TAS1R3 and mGluInternational journal of molecular sciences · 2023Article
- Phosphorylation status of Bβ subunit acts as a switch to regulate the function of phosphatase PP2A in ethylene-mediated root growth inhibition.The New phytologist · 2022Article
- Measurement of Protein-Protein Interaction Dynamics Using Microfluidics and Particle Diffusometry.Analytical chemistry · 2022Article
- CRISPR/Cas9-mediated P-CR domain-specific engineering of CESA4 heterodimerization capacity alters cell wall architecture and improves saccharification efficiency in poplar.Plant biotechnology journal · 2022Article
- Improving the Identification and Coverage of Plant Transmembrane Proteins inFrontiers in plant science · 2020Article
- Perception of double-stranded RNA in plant antiviral immunity.Molecular plant pathology · 2019Review
- Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence.Plant biotechnology journal · 2019Article
- Noncanonical ATG8-ABS3 interaction controls senescence in plants.Nature plants · 2019Article
- VAPYRIN Marks an Endosomal Trafficking Compartment Involved in Arbuscular Mycorrhizal Symbiosis.Frontiers in plant science · 2019Article
- RIN4 recruits the exocyst subunit EXO70B1 to the plasma membrane.Journal of experimental botany · 2017Article
- Split-ubiquitin yeast two-hybrid interaction reveals a novel interaction between a natural resistance associated macrophage protein and a membrane bound thioredoxin in Brassica juncea.Plant molecular biology · 2016Article
- Techniques for the Analysis of Protein-Protein Interactions in Vivo.Plant physiology · 2016Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of cell-surface receptor dynamics is critical for understanding how cells sense and respond to changing environments. Therefore, elucidating the mechanisms by which signals are perceived and communicated into the cell is necessary to understand immunity, development, and stress. Challenges in testing interactions of membrane-bound proteins include their dynamic nature, their abundance, and the complex dual environment (lipid/soluble) in which they reside. Co-Immunoprecipitation (Co-IP) of tagged membrane proteins is a widely used approach to test protein-protein interaction in vivo. In this protocol we present a method to perform Co-IP using enriched membrane proteins in isolated microsomal fractions. The different variations of this protocol are highlighted, including recommendations and troubleshooting guides in order to optimize its application. This Co-IP protocol has been developed to test the interaction of receptor-like kinases, their interacting partners, and peptide ligands in stable Arabidopsis thaliana lines, but can be modified to test interactions in transiently expressed proteins in tobacco, and potentially in other plant models, or scaled for large-scale protein-protein interactions at the membrane.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.