ArticleBiotechnology progress
Characterizing the release of bioactive N-glycans from dairy products by a novel endo-β-N-acetylglucosaminidase.
Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Novel Endo-β-Foods (Basel, Switzerland) · 2025Article
- Identification and comparison ofFood chemistry: X · 2025Article
- Bovine Colostrum in Pediatric Nutrition and Health.Nutrients · 2024Review
- Substitutive Effects of Milk vs. Vegetable Milk on the Human Gut Microbiota and Implications for Human Health.Nutrients · 2024Review
- Exploring the impact of colostrum supplementation on athletes: a comprehensive analysis of clinical trials and diverse properties.Frontiers in immunology · 2024Review
- The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties.Journal of agricultural and food chemistry · 2023Review
- Bovine colostrum and its potential contributions for treatment and prevention of COVID-19.Frontiers in immunology · 2023Review
- Role of milk glycome in prevention, treatment, and recovery of COVID-19.Frontiers in nutrition · 2022Review
- Immobilization of a Bifidobacterial Endo-Frontiers in bioengineering and biotechnology · 2022Article
- Bioactive compounds, nutritional profile and health benefits of colostrum: a review.Food production, processing and nutrition · 2022Review
- Potential applications of recombinant bifidobacterial proteins in the food industry, biomedicine, process innovation and glycobiology.Food science and biotechnology · 2021Review
- Bovine Colostrum and Its Potential for Human Health and Nutrition.Frontiers in nutrition · 2021Review
- Potential Applications of Endo-β-Frontiers in nutrition · 2021Review
- In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile.Journal of agricultural and food chemistry · 2020Article
- Structural insights of two novel N-acetyl-glucosaminidase enzymes through in silico methods.Turkish journal of chemistry · 2020Article
- Immobilization of an Endo-β-Catalysts (Basel, Switzerland) · 2018Article
- Characterization of recombinant human lactoferrin N-glycans expressed in the milk of transgenic cows.PloS one · 2017Article
- Recent advances in immobilization strategies for glycosidases.Biotechnology progress · 2017Review
- Oligosaccharides Released from Milk Glycoproteins Are Selective Growth Substrates for Infant-Associated Bifidobacteria.Applied and environmental microbiology · 2016Article
- A novel endo-β-N-acetylglucosaminidase releases specific N-glycans depending on different reaction conditions.Biotechnology progressArticle
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Endo-β-N-acetylglucosaminidase isolated from B. infantis ATCC 15697 (EndoBI-1) is a novel enzyme that cleaves N-N'-diacetyl chitobiose moieties found in the N-glycan core of high mannose, hybrid, and complex N-glycans. These conjugated N-glycans are recently shown as a new prebiotic source that stimulates the growth of a key infant gut microbe, Bifidobacterium longum subsp. Infantis. The effects of pH (4.45-8.45), temperature (27.5-77.5°C), reaction time (15-475 min), and enzyme/protein ratio (1:3,000-1:333) were evaluated on the release of N-glycans from bovine colostrum whey by EndoBI-1. A central composite design was used, including a two-level factorial design (2(4)) with four center points and eight axial points. In general, low pH values, longer reaction times, higher enzyme/protein ratio, and temperatures around 52°C resulted in the highest yield. The results demonstrated that bovine colostrum whey, considered to be a by/waste product, can be used as a glycan source with a yield of 20 mg N-glycan/g total protein under optimal conditions for the ranges investigated. Importantly, these processing conditions are suitable to be incorporated into routine dairy processing activities, opening the door for an entirely new class of products (released bioactive glycans and glycan-free milk). The new enzyme's activity was also compared with a commercially available enzyme, showing that EndoBI-1 is more active on native proteins than PNGase F and can be efficiently used during pasteurization, streamlining its integration into existing processing strategies.
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