ArticleBiotechnology journal2015
High-content imaging with micropatterned multiwell plates reveals influence of cell geometry and cytoskeleton on chromatin dynamics.
Article in Biotechnology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- RosetteArraybioRxiv : the preprint server for biology · 2024Article
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Label-Free Imaging to Track Reprogramming of Human Somatic Cells.GEN biotechnology · 2022Article
- Morphological features of single cells enable accurate automated classification of cancer from non-cancer cell lines.Scientific reports · 2021Article
- Design of efficacious somatic cell genome editing strategies for recessive and polygenic diseases.Nature communications · 2020Article
- Tracking and Predicting Human Somatic Cell Reprogramming Using Nuclear Characteristics.Biophysical journal · 2020Article
- A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing.Nature nanotechnology · 2019Article
- The Driving Force: Nuclear Mechanotransduction in Cellular Function, Fate, and Disease.Annual review of biomedical engineering · 2019Review
- Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation.Lab on a chip · 2019Article
- Wound Healing Coordinates Actin Architectures to Regulate Mechanical Work.Nature physics · 2019Article
- Bioengineering Solutions for Manufacturing Challenges in CAR T Cells.Biotechnology journal · 2018Review
- Razor-printed sticker microdevices for cell-based applications.Lab on a chip · 2018Article
- The component formula ofOncotarget · 2017Article
- Manufacturing Cell Therapies Using Engineered Biomaterials.Trends in biotechnology · 2017Review
- Valve interstitial cell contractile strength and metabolic state are dependent on its shape.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells.Acta biomaterialia · 2016Article
- High-Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells.Stem cell reports · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Understanding the mechanisms underpinning cellular responses to microenvironmental cues requires tight control not only of the complex milieu of soluble signaling factors, extracellular matrix (ECM) connections and cell-cell contacts within cell culture, but also of the biophysics of human cells. Advances in biomaterial fabrication technologies have recently facilitated detailed examination of cellular biophysics and revealed that constraints on cell geometry arising from the cellular microenvironment influence a wide variety of human cell behaviors. Here, we create an in vitro platform capable of precise and independent control of biochemical and biophysical microenvironmental cues by adapting microcontact printing technology into the format of standard six- to 96-well plates to create MicroContact Printed Well Plates (μCP Well Plates). Automated high-content imaging of human cells seeded on μCP Well Plates revealed tight, highly consistent control of single-cell geometry, cytoskeletal organization, and nuclear elongation. Detailed subcellular imaging of the actin cytoskeleton and chromatin within live human fibroblasts on μCP Well Plates was then used to describe a new relationship between cellular geometry and chromatin dynamics. In summary, the μCP Well Plate platform is an enabling high-content screening technology for human cell biology and cellular engineering efforts that seek to identify key biochemical and biophysical cues in the cellular microenvironment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.