ArticleMethods in enzymology2015
Affinity Purification of Protein Complexes Using TAP Tags.
Article in Methods in enzymology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Proteomic and glycosylation biomarkers in rheumatoid arthritis: advancing early diagnosis and precision therapy.Clinical proteomics · 2026Review
- Tandem Affinity Purification and Mass-Spectrometric Analysis of FACT and Associated Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Fuzzy RNA recognition by the Trypanosoma brucei editosome.Nucleic acids research · 2022Article
- Tandem affinity purification protocol for isolation of protein complexes fromSTAR protocols · 2022Article
- Trypanosomatid, fluorescence-basedBio-protocol · 2021Article
- Analyzing editosome function in high-throughput.Nucleic acids research · 2020Article
- Article
- Yck1 casein kinase I regulates the activity and phosphorylation of Pah1 phosphatidate phosphatase fromThe Journal of biological chemistry · 2019Article
- Mapping transcriptome-wide protein-RNA interactions to elucidate RNA regulatory programs.Quantitative biology (Beijing, China) · 2018Article
- Advances and challenges in the detection of transcriptome-wide protein-RNA interactions.Wiley interdisciplinary reviews. RNA · 2018Review
- Single-step purification of intrinsic protein complexes inMethodsX · 2018Article
- How to prove the existence of metabolons?Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2018Review
- Development of Series of Affinity Tags in Streptomyces.Scientific reports · 2017Article
- The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis.RNA (New York, N.Y.) · 2016Article
- Disturbed macro-connectivity in schizophrenia linked to oligodendrocyte dysfunction: from structural findings to molecules.NPJ schizophrenia · 2015Review
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Authors and funding
2 authors.
Funding
Abstract
This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage site. This is a powerful technique for rapid purification of protein complexes and the analysis of their stoichiometric composition, posttranslational modifications, structure, and functional activities.
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Registered trials
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