ArticleJournal of functional biomaterials2015
Human Keratoconus Cell Contractility is Mediated by Transforming Growth Factor-Beta Isoforms.
Article in Journal of functional biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- Systematically Displaying the Pathogenesis of KeratoconusFrontiers in medicine · 2021Pooled it
- Update on the Physiopathology of Keratoconus.Medical sciences (Basel, Switzerland) · 2026Review
- Mechanical Strain of Corneal Epithelium Influences the Expression of Genes Implicated in Keratoconus.Investigative ophthalmology & visual science · 2025Article
- Matrix glycosaminoglycans and proteoglycans in human cornea organoids and similarities with fetal corneal stages.The ocular surface · 2025Article
- The impact of UV cross-linking on corneal stromal cell migration, differentiation and patterning.Experimental eye research · 2023Article
- Unravelling the Impact of Cyclic Mechanical Stretch in Keratoconus-A Transcriptomic Profiling Study.International journal of molecular sciences · 2023Article
- Cellular Contractility Profiles of Human Diabetic Corneal Stromal Cells.Analytical cellular pathology (Amsterdam) · 2021Article
- Transcriptional profiling of corneal stromal cells derived from patients with keratoconus.Scientific reports · 2019Article
- Tranilast inhibits TGF-β-induced collagen gel contraction mediated by human corneal fibroblasts.International journal of ophthalmology · 2018Article
- Human in vitro Model Reveals the Effects of Collagen Cross-linking on Keratoconus Pathogenesis.Scientific reports · 2017Article
- Collagen synthesis disruption and downregulation of core elements of TGF-β, Hippo, and Wnt pathways in keratoconus corneas.European journal of human genetics : EJHG · 2017Article
- Analysis of sphingolipids in human corneal fibroblasts from normal and keratoconus patients.Journal of lipid research · 2017Article
- Organoids: A historical perspective of thinking in three dimensions.The Journal of cell biology · 2017Review
- Role of corneal collagen fibrils in corneal disorders and related pathological conditions.International journal of ophthalmology · 2017Review
- Pathophysiology of Keratoconus: What Do We Know Today.The open ophthalmology journal · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Keratoconus (KC) is a progressive disease linked to defects in the structural components of the corneal stroma. The extracellular matrix (ECM) is secreted and assembled by corneal keratocytes and regulated by transforming growth factor-β (TGF-β). We have previously identified alterations in the TGF-β pathway in human keratoconus cells (HKCs) compared to normal corneal fibroblasts (HCFs). In our current study, we seeded HKCs and HCFs in 3D-collagen gels to identify variations in contractility, and expression of matrix metalloproteases (MMPs) by HKCs in response the TGF-β isoforms. HKCs showed delayed contractility with decreased Collagen I:Collagen V ratios. TGF-β1 significantly increased ECM contraction, Collagen I, and Collagen V expression by HKCs. We also found that HKCs have significantly decreased Collagen I:Collagen III ratios suggesting a potential link to altered collagen isoform expression in KC. Our findings show that HKCs have significant variations in collagen secretion in a 3D collagen gel and have delayed contraction of the matrix compared to HCFs. For the first time, we utilize a collagen gel model to characterize the contractility and MMP expression by HKCs that may contribute to the pathobiology of KC.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.