ArticleInternational journal of circumpolar health2015
Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in diarrhoeic patients in the Qikiqtani Region, Nunavut, Canada.
Article in International journal of circumpolar health, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.
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Who cites it
15 citing papers in PubMed, 2 syntheses or guidelines pooled it, 33 citations in OpenAlex.
- Prevalence of human cryptosporidiosis in the Americas: systematic review and meta-analysis.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Pooled it
- Anthroponotic transmission of Cryptosporidium parvum predominates in countries with poorer sanitation: a systematic review and meta-analysis.Parasites & vectors · 2019Pooled it
- Prevalence and molecular characterization ofFood and waterborne parasitology · 2025Article
- Occurrence and Multi-Locus Genotyping ofAnimals : an open access journal from MDPI · 2025Article
- Metagenomic detection of protozoan parasites on leafy greens aided by a rapid and efficient DNA extraction protocol.Frontiers in microbiology · 2025Article
- Intestinal Protozoan Infections in Patients with Diarrhea - Shanghai Municipality, Zhenjiang City, and Danyang City, China, 2011-2015 and 2019-2021.China CDC weekly · 2022Article
- Molecular characterization of Cryptosporidium isolates from humans in Ontario, Canada.Parasites & vectors · 2021Article
- The controversies surroundingCurrent research in parasitology & vector-borne diseases · 2021Review
- The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil.Biomedica : revista del Instituto Nacional de Salud · 2020Article
- First report of Cryptosporidium viatorum and Cryptosporidium occultus in humans in China, and of the unique novel C. viatorum subtype XVaA3h.BMC infectious diseases · 2020Article
- Water safety plans as a tool for drinking water regulatory frameworks in Arctic communities.Environmental science and pollution research international · 2018Article
- Prevalence of Cryptosporidium, Blastocystis, and other opportunistic infections in patients with primary and acquired immunodeficiency.Parasitology research · 2018Article
- Practical Guidance for Clinical Microbiology Laboratories: Laboratory Diagnosis of Parasites from the Gastrointestinal TractClinical microbiology reviews · 2018Review
- Estimates of the burden of illness for eight enteric pathogens associated with animal contact in Canada.Epidemiology and infection · 2017Article
- Cryptosporidium hominis Is a Newly Recognized Pathogen in the Arctic Region of Nunavik, Canada: Molecular Characterization of an Outbreak.PLoS neglected tropical diseases · 2016Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough the prevalences of infection with the protozoan parasites Cryptosporidium spp. and Giardia duodenalis in humans appear to be relatively high in the Canadian North, their transmission patterns are poorly understood.
objectiveTo determine the detection rate and the molecular characteristics of Cryptosporidium spp. and Giardia duodenalis in diarrhoeic patients in the Qikiqtani (Baffin Island) Region of Nunavut, Canada, in order to better understand the burden of illness and the potential mechanisms of transmission. STUDY DESIGN/
methodsDiarrhoeal stool specimens (n=108) submitted to the Qikiqtani General Hospital for clinical testing were also tested for the presence of Cryptosporidium spp. and Giardia duodenalis using epifluorescence microscopy and polymerase chain reaction (PCR). DNA sequencing and restriction fragment length polymorphism (RFLP) analyses were performed on PCR-positive specimens to determine the species, genotypes and sub-genotypes of the parasites.
resultsCryptosporidium was detected in 15.7% of the diarrhoeic patients, while Giardia was detected in 4.6%. DNA sequencing of a fragment of the small subunit rRNA gene indicated that all of the Cryptosporidium amplicons had a 100% homology to C. parvum, and a gp60 assay showed that all aligned with C. parvum sub-genotype IIa. Microsatellite analysis revealed 3 cases of sub-genotype IIaA15G2R1, 2 of IIaA15G1R and 1 case each of sub-genotypes IIaA16G1R1 and IIaA15R1. For Giardia, results based on the amplification of both the 16S rRNA gene and the gdh gene were generally in agreement, and both DNA sequencing and RFLP demonstrated the presence of the G. duodenalis Assemblage B genotype.
conclusionsBoth C. parvum and G. duodenalis Assemblage B were present in human diarrhoeal stool specimens from Nunavut, which was suggestive of zoonotic transmission, although human-to-human transmission cannot be ruled out. To fully understand the public health significance of the different Cryptosporidium and Giardia species and genotypes in diarrhoeic patients, it will be imperative to establish the extent of genetic diversity within these parasites through comprehensive studies of the molecular epidemiology of cryptosporidiosis and giardiasis in the Nunavut region.
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