ArticleDevelopment (Cambridge, England)2015
A rapid, membrane-dependent pathway directs furrow formation through RalA in the early Drosophila embryo.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- GCL pruning of PIP3 establishes the soma-germline boundary.The Journal of cell biology · 2026Article
- ER shaping proteins guide spindle elongation and division during rapid cleavage mitoses.bioRxiv : the preprint server for biology · 2026Article
- Bitesize bundles F-actin and influences actin remodeling in syncytial Drosophila embryo development.The Journal of cell biology · 2026Article
- MIM triggers formin to Arp2/3-based actin assembly in membrane remodeling in Drosophila embryos.The Journal of cell biology · 2026Article
- Centrosome-organized plasma membrane infoldings linked to growth of a cortical actin domain.The Journal of cell biology · 2024Article
- Reshaping the Syncytial Drosophila Embryo with Cortical Actin Networks: Four Main Steps of Early Development.Results and problems in cell differentiation · 2024Review
- Adhesion and Polarity protein distribution-regulates hexagon dominated plasma membrane organization in Drosophila blastoderm embryos.Genetics · 2023Article
- Dysregulation of the endoplasmic reticulum blocks recruitment of centrosome-associated proteins resulting in mitotic failure.Development (Cambridge, England) · 2023Article
- A Rab39-Klp98A-Rab35 endocytic recycling pathway is essential for rapid Golgi-dependent furrow ingression.Development (Cambridge, England) · 2023Article
- Membrane-actin interactions in morphogenesis: Lessons learned from Drosophila cellularization.Seminars in cell & developmental biology · 2023Review
- Lipid Polarization during Cytokinesis.Cells · 2022Review
- Pseudocleavage furrows restrict plasma membrane-associated PH domain in syncytial Drosophila embryos.Biophysical journal · 2022Article
- Review
- Combinatorial deployment of F-actin regulators to build complex 3D actin structures in vivo.eLife · 2021Article
- Article
- Ral function in muscle is required for flight maintenance inSmall GTPases · 2020Article
- Par-1 controls the composition and growth of cortical actin caps duringThe Journal of cell biology · 2019Article
- Key roles of Arf small G proteins and biosynthetic trafficking for animal development.Small GTPases · 2019Review
- Differentially-dimensioned furrow formation by zygotic gene expression and the MBT.PLoS genetics · 2018Article
- Analysis of mitochondrial organization and function in the Drosophila blastoderm embryo.Scientific reports · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Plasma membrane furrow formation is crucial in cell division and cytokinesis. Furrow formation in early syncytial Drosophila embryos is exceptionally rapid, with furrows forming in as little as 3.75 min. Here, we use 4D imaging to identify furrow formation, stabilization, and regression periods, and identify a rapid, membrane-dependent pathway that is essential for plasma membrane furrow formation in vivo. Myosin II function is thought to provide the ingression force for cytokinetic furrows, but the role of membrane trafficking pathways in guiding furrow formation is less clear. We demonstrate that a membrane trafficking pathway centered on Ras-like protein A (RalA) is required for fast furrow ingression in the early fly embryo. RalA function is absolutely required for furrow formation and initiation. In the absence of RalA and furrow function, chromosomal segregation is aberrant and polyploid nuclei are observed. RalA localizes to syncytial furrows, and mediates the movement of exocytic vesicles to the plasma membrane. Sec5, which is an exocyst complex subunit and localizes to ingressing furrows in wild-type embryos, becomes punctate and loses its cortical association in the absence of RalA function. Rab8 also fails to traffic to the plasma membrane and accumulates aberrantly in the cytoplasm in RalA disrupted embryos. RalA localization precedes F-actin recruitment to the furrow tip, suggesting that membrane trafficking might function upstream of cytoskeletal remodeling. These studies identify a pathway, which stretches from Rab8 to RalA and the exocyst complex, that mediates rapid furrow formation in early Drosophila embryos.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.